摘要
目的探讨辣椒素对1型糖尿病大鼠糖代谢的影响及作用机制。方法 (1)普通大鼠设为低、中、高剂量组和空白组(n=8),剂量组分别灌胃3、6、9 mg/(kg·bw)剂量的辣椒素28 d后,检测其餐后血糖,肠道淀粉酶和葡萄糖苷酶活性;(2)建立链脲佐菌素诱导糖尿病大鼠模型,将其分为低、中、高剂量组,空白组和模型组(n=8),剂量组分别灌胃3、6、9 mg/(kg·bw)剂量辣椒素28 d后,依次检测其餐后血糖,消化酶的活性,葡萄糖耐量,血清胰岛素,肝糖元和糖化血清蛋白等与糖代谢相关指标。结果中高剂量辣椒素可显著抑制正常大鼠肠道淀粉酶和葡萄糖苷酶的活性,降低负荷(葡萄糖、蔗糖和淀粉)的餐后血糖含量,以中剂量最佳。且高剂量辣椒素可显著降低糖尿病大鼠糖化血清蛋白。结论辣椒素的降血糖机制可能通过两种途径实现:(1)通过辣椒素对肠道相关酶活性的抑制以及降低小肠对碳水化合物吸收能力,实现对肠内葡萄糖吸收的干扰;(2)刺激胰岛素的分泌和肝糖原合成,抑制糖异生,从而达到降低血糖的目的。
Objective To examine the effect of capsaicin on glucose metabolism in type 1 diabetes rats and its potential mechanism.Methods （1） Normal rats were divided into three treatment groups with different dosages of capsaicin, and one blank control group （n=8）. In the treatment groups, rats were treated by 3, 6, 9 mg/（kg·bw） of capsaicin respectively for 28 days. Then, postprandial blood glucose, intestinal amylase and glycosidase enzymes were observed; （2） The diabetic rats model was induced by streptozotocin, and the rats were divided into three treatment groups, blank control group and model group （n=8）. In the treatment groups, rats were fed capsaicin （3, 6, 9 mg/kg·bw） for 28 days. Subsequently, the levels of postprandial blood sugar, glucose tolerance, glycosylated serum protein, hepatic glycogen, serum insulin and the activity of digestive enzymes were examined.Results Medium and high dosages of capsaicin significantly inhibited the activity of amylase and glucosaccharase enzymes in rat intestine, and significantly reduced the postprandial blood sugar levels in normal rats. The medium dosage of capsaicin had the greater effects than the high dosage of capsaicin. Moreover, the high dosage of capsaicin significantly reduced the level of serum glycated albumin.Conclusion The decrease of blood glucose by capsaicin may be achieved by two ways：（1） Inhibiting the enzyme activity and intestinal absorption of carbohydrate; （2） Increasing insulin secretion and hepatic glycogen synthesis.
出处
《营养学报》
CAS
CSCD
北大核心
2017年第1期76-80,85,共6页
Acta Nutrimenta Sinica
基金
国家自然科学基金项目(No.31471581)
关键词
辣椒素
糖尿病大鼠
糖代谢
糖耐量
capsaicin
diabetic rats
sugar metabolism
glucose tolerance
