摘要
为促进猪场污水治理,研究微藻净化对猪场污水菌群的影响,将小球藻接种于含60%沼液与40%水的猪场污水进行批式模式培养,循环三次后将小球藻液离心,检测猪场污水、小球藻液以及离心后上清液中的水质指标及菌群变化情况。菌群采用16S rDNA克隆文库方法检测。结果显示,和猪场污水相比,小球藻液及上清液中的化学耗氧量(COD)、总氮、氨氮、总磷、铜、锌等含量极显著降低(P<0.01)。除未知菌群外,猪场污水中的主要菌群为Firmicutes群和Bellilinea群,含量分别为12.25%和11.22%;小球藻液中主要菌群为小球藻和Cytophaga群,含量分别为42.42%和12.12%;上清液中的主要菌群为Dyadobacter、Cytophaga、Algoriphagus群和Pedobacter群,含量分别为25.00%、14.00%、11.00%和10.00%。猪场污水和小球藻液中未发现共存的微生物,小球藻液与上清液中皆含有Cytophaga群和Dyadobacter、Pseudomonas、Flavobacterium、Algoriphagus、Flexibacter群。表明接种小球藻可以净化猪场污水中的氨氮、总磷以及重金属,改变污水中的菌群,污水净化可能是小球藻和其共存菌共同作用的结果。
In order to promote wastewater treatment,the effects of purification with microalgae on microflora of swine wastewater were studied. The Chlorella vulgaris was inoculated in swine wastewater with 60% of biogas slurry and 40% of water,and then the chlorella solution was centrifuged after cycling culturing for three times with batch mode. The water quality indices were measured in swine wastewater,chlorella solution and supernatant solution after centrifugation,and the microflora changes were detected with 16 S r DNA clone library technology. The results showed that compared with swine wastewater,the chemical oxygen demand（ COD）,total nitrogen（ TN）,ammonia nitrogen（ NH3-N）,total phosphor（ TP）,copper and zinc concentrations significantly decreased in chlorella solution and supernatant solution（ P〈0. 01）. Besides uncultured microflora,theprincipal microflora in swine wastewater were Firmicutes bacterium（ 12. 25%） and Bellilinea sp.（ 11. 22%）,those in chlorella solution were Chlorella（ 42. 42%） and Cytophaga sp.（ 12. 12%）,and those in supernatant solution were Dyadobacter sp.（ 25. 00%）,Cytophaga sp.（ 14. 00%）,Algoriphagus sp.（ 11. 00%） and Pedobacter sp.（ 10. 00%）. There was no coexisting microflora in swine wastewater and chlorella solution,while Cytophaga sp.,Dyadobacter sp.,Pseudomonas sp.,Flavobacterium sp.,Algoriphagus sp. and Flexibacter sp.existed in both chlorella solution and supernatant solution. In summary,inoculating Chlorella could decrease the NH3-N,TP and heavy metal contents in swine wastewater and change the microflora in wastewater. The purification of swine wastewater might be the result of Chlorella and its coexisting microflora.
出处
《山东农业科学》
2017年第2期93-98,共6页
Shandong Agricultural Sciences
基金
环保部水体污染控制与治理科技重大专项(2013ZX07103006-007)
山东省农业重大应用技术创新项目[鲁财农指(2016)36号]
