摘要
【目的】采用高效液相色谱法(HPLC)、液相色谱—质谱联用技术(HPLC-MS)研究姜黄素的碱稳定性及其碱降解产物,制定姜黄清脂片中碱溶工艺的参数以制备质量稳定的姜黄清脂片。【方法】以乙腈—体积分数4%冰醋酸(体积比48∶52)为流动相,430 nm为检测波长,测定姜黄素在碱液中的稳定性;HPLC-MS采用电喷雾离子源和四级杆飞行时间质谱,100~2 000 m/z内扫描研究其碱降解产物。【结果】姜黄素在碱液中温度越高降解越快,当温度低于20℃时,降解缓慢,温度为80℃时,2 h降解完全,推测可能的降解产物为对羟基苯甲醛、香草醛、对香豆酸、阿魏酸等。【结论】姜黄素类化合物在碱液中不稳定,应控制碱溶工艺的温度在20℃以下,制备得到的姜黄清脂片含量高,降解产物少。
Objective To study the stability of alkaline and degraded products of curcumin by high performance liquid chromatography（HPLC）and HPLC-mass spectrometry（MS）for the optimization of alkaline-dissolved process parameters of the Jianghuang Qingzhi Tablets and for the quality control of the tablets. Methods HPLC was performed for the determination of the alkaline-dissolved stability of curcumin with acetonitrile-acetic acid at volume coefficient of 4%（48 ∶ 52） as mobile phase, the detection wavelength being 430 nm. The alkaline-degraded products were tested by HPLC-MS assembling with electron spray ionization（ESI）and quadrupole timeof-flight（Q-TOF）in the scan range of 100-2 000 m/z. Results The degradation of curcumin in the alkaline solution was increased with the temperature. When the temperature was below 20 ℃,the degradation slowed down,while when the temperature reached to 80 ℃,curcumin was degraded completely within 2 h. The probable degradation products in the alkaline solution were p-hydroxy benzaldehyde,vanillin,p-coumaric acid, ferulic acid,et al.Conclusion Curcumin compounds are instable in aqueous alkali. To obtain the high-quality of Jianghuang Qingzhi Tablets with high content of curcumin and less degraded products, the alkaline-solution temperature should be controlled below 20 ℃.
出处
《广州中医药大学学报》
CAS
2017年第2期260-263,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
关键词
姜黄素
姜黄清脂片/生产和制备
降解产物
HPLC-MS
色谱法
高压液相
curcumin
Jianghuang Qingzhi Tablets/production and preparation
degradation products
HPLC-MS
chromatography
high performance liquid
