摘要
目的:研究美洲大蠊多肽提取物诱导人肝癌细胞SMMC-7721凋亡及其分子机制。方法:采用不同质量浓度的美洲大蠊多肽提取物作用于SMMC-7721,以Cell Counting Kit-8(CCK-8)法检测细胞抑制率;Hoechst33342/PI与Annexin V-FITC/PI双染法相结合检测SMMC-7721细胞的凋亡;蛋白免疫印迹(Western blotting)法检测细胞凋亡相关因子Bcl-2和Bax蛋白表达。结果:不同质量浓度的美洲大蠊多肽提取物可抑制SMMC-7721细胞的增殖,诱导其凋亡,呈一定的量效关系;Western Blot法显示Bax表达增多,Bcl-2蛋白表达减少,Bcl-2/Bax比值降低。结论:美洲大蠊多肽提取物可明显诱导SMMC-7721细胞凋亡,抑制其增殖,其机制可能与下调Bcl-2蛋白表达,上调Bax蛋白表达,降低Bcl-2/Bax比值有关。
OBJECTIVE To study the mechanism of cell apoptosis and proliferation induced by Periplaneta americana polypeptide extract in human hepatocellular carcinoma SMMC-7721 cells. METHODS SMMC-7721 cells were treated with Periplaneta americana polypeptide extract at different concentrations. The growth inhibitory rates of SMMC-7721 cells were measured by CCK-8 assay. Hoechst33342/PI and Annexin V-FITC/PI assays were used to detect apoptosis in SMMC-7721 cells. Bcl-2 and Bax expression were investigated by Western blot. RESULTS Cell proliferation was inhibited and cell apoptosis was induced significantly in SMMC-7721 cells by Periplaneta americana polypeptide extract in a certain dose dependent manner. Western blot showed that Bax expression was upregulated,Bcl-2 expression downregulated,ratio of Bcl-2/Bax decreased. CONCLUSION Periplaneta american polypeptide extract can obviously promote apoptosis and inhibit proliferation of SMMC-7721 cells,by reducing protein expression of Bcl-2,increasing protein expression of Bax and reducing Bcl-2/Bax ratio.
出处
《中国医院药学杂志》
CAS
北大核心
2017年第4期328-332,共5页
Chinese Journal of Hospital Pharmacy
基金
四川省科技厅科技计划项目(编号:2015JY0098)
