摘要
目的比较研究三大产地5个品种金银花及其掺杂叶活性成分含量及指纹图谱相似度,为其优质优价采购以及资源综合利用提供参考。方法采集山东大毛花、鸡爪花和四季花、河南豫封一号和河北巨花一号不同茬次的金银花,分离其掺杂叶,测定并比较92份样品中活性成分的含量和HPLC指纹图谱相似度。结果 5个品种金银花活性成分含量均随采收时间呈先降后升趋势,头茬花含量最高,二茬花含量最低;绿原酸含量头茬花大毛花最高,四茬花巨花一号最高,木犀草苷含量不同品种间未体现差异;不同品种金银花HPLC指纹图谱相似度在0.9以上,豫封一号与其它品种相似度相对较低;掺杂叶的绿原酸和木犀草苷含量均高于金银花,HPLC指纹图谱与金银花相似度为0.815~0.892。结论头茬花和四茬花品质较高,头茬花以大毛花质量为优,四茬花以巨花一号质量较佳;掺杂叶不能与金银花混用,但其活性成分含量高于金银花。本研究为金银花品质评价和掺杂叶的开发利用提供了科学依据。
OBJECTIVE To comprehensive and systematic study the effective components of different stubbles in honey- suckle and doped leaves from the main cultivars of the main producing areas, and to provide reference for procurement of high quality and low price and for comprehensive utilization of resources. METHODS Collect 92 samples of honeysuckle and doped leaves, which from 5 cultivars of the three main producing areas of Shandong, Henan and Hebei. Mensurate the content of the index components in the sample and the high performance liquid chromatographic fingerprint. RESULTS There were differ- ences in the contents of the index components of different stubble. The highest content of the first crop, the lowest of the two. The two crop luteolin content is lower than the standard of Chinese Pharmacopoeia. The content of chlorogenic acid in the first crop was the highest in the Damaohua, and in four crop was the highest in the Juhuayihao. HPLC fingerprint similarity of hon- eysuckle was more than 0.9. The content of the index components of the doped leaves was higher than that of the honeysuckle. The HPLC fingerprint similarity of the doped leaves and of the honeysuckle was between 0. 815-0. 892. CONCLUSION The first and four stubble quality is higher in three main producing areas of five varieties of honeysuckle. Damaohua＇ s quality is ex- cellent in the first stubble, and Juhuayihao＇s quality is excellent in the four stubble. Chemical composition between doped leaves and honeysuckle are different. The index component content of doped leaves is higher than honeysuckle. This study provided the scientific basis for the quality evaluation of honeysuckle, and the development and utilization of the doped leaves.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2017年第1期87-92,共6页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省中药优势学科Ⅱ期建设(ysxk-2014)
江苏省中药资源产业化过程协同创新中心重点课题项目(ZDXMHT-1-5)
关键词
金银花
掺杂叶
绿原酸
木犀草苷
指纹图谱
Lonicera japonica
doped leaves
chlorogenic acid
galuteolin
fingerprint
