摘要
目的研究海南槟榔提取物的抗衰老作用。方法昆明种小鼠150只,分为正常组,模型组,石杉碱甲组,槟榔提取物高、低剂量组,每组30只。除正常组外,其余小鼠每日上午皮下注射D-半乳糖800 mg/kg,连续8周,建立衰老模型。石杉碱甲组和槟榔提取物高、低剂量组分别预防性灌胃给予石杉碱甲(0.4 mg/kg)、海南槟榔提取物高(500 mg/kg)、低剂量(170 mg/kg)。8周后,Morris水迷宫检测衰老小鼠的学习记忆能力;处死小鼠,取海马组织,测定SOD、SDH、GSH-Px的活性和MDA含量;取大脑皮层,10%甲醛固定,HE染色,观察大脑皮层神经细胞的组织学变化。结果与正常组比较,模型组小鼠的逃避潜伏期明显增加,跨越平台次数明显降低(P<0.01),海马组织MDA含量增加(P<0.01),SOD(P<0.05)、SDH(P<0.01)和GSH-Px(P<0.05)的活性明显增加,大脑皮质神经细胞深染,排列紊乱。槟榔提取物高、低剂量均能显著降低衰老小鼠逃避潜伏期,增加跨越平台次数(P<0.01);增强海马组织SOD(P<0.05)、SDH活性(P<0.01),降低MDA含量(P<0.01),低剂量还能增强GSH-Px的活性(P<0.05),改善衰老小鼠大脑皮质神经细胞的组织学变化。结论海南槟榔提取物能改善衰老小鼠的学习记忆能力、脑组织抗氧化能力和组织学改变,有抗衰老作用。
Objective To investigate the anti-ageing effects of betel nut extract grown in Hainan island.Methods A total of 150 Kunming mice were divided into 5 groups,that is,normal control,model,huperzine a,high- and low-dosed betel nut extract groups,with 30 mice in each group. Ageing mice models were established by subcutaneous injection of D-galactose at a dose of 800 mg/kg every morning for 8 weeks.And 0.4 mg/kg huperzine a and 500 and 170 mg/kg betel nut extracts were administered intragastrically.In 8 weeks later,learning-memory ability of ageing mice was tested by Morris water maze test.After the mice were sacrificed and their hippocampal tissues were collected to detect the activities of SOD,SDH,GSH-Px and MDA. HE staining was used to observe the morphological changes of the nerve cells in the cerebral cortex after 10%formaldehyde fixation.Results Compared with the normal control group,the mice in the model group exhibited longer escape latency and lower platform-crossing times（P〈0.01）,increased content of MDA（P〈0.01） and enhanced activities of SOD（P〈0.05）,SDH（P〈0.01） and GSH-Px（P〈0.05） in the hippocampal tissues,and deeply dyed neural cells with irregularalignment.High- and low-dosed betel nut extract shortened escape latency and increased platform-crossing times in Morris water maze test（P〈0.01）; increased the activities of SOD（P〈0.05）,SDH（P〈0.01）,but decreased the amount of MDA（P〈0.01） in the in the hippocampus. What＇s more,the low- dosed extract also could enhance the activity of GSH- Px（P〈0.05）,and improve histological changes in ageing mice. Conclusion Betel nut extractgrown in Hainan island exerts the anti-ageing effects by enhancing learning-memory,and promoting brain anti-oxidative ability and histological changes in ageing mice.
出处
《中国热带医学》
CAS
2017年第2期123-125,共3页
China Tropical Medicine
基金
海南省重点科技计划项目(No.ZDXM20120050)
关键词
槟榔
提取物
抗衰老
betel nut
extract
anti-ageing
