摘要
里氏木霉(Trichoderma reesei)QM6a为重要的产纤维素酶工业生产菌株,为明确其内存在的分泌蛋白,对该菌分泌蛋白进行预测,并明确其特征。利用SignalP、ProtComp等5个软件对该菌中9 143条蛋白质序列进行分泌蛋白预测,并对上述分泌蛋白的氨基酸大小分布情况、信号肽长度及其切割位点等性质进行分析。里氏木霉中含有分泌蛋白356个,其氨基酸长度、信号肽长度与其他植物病原菌不同;信号肽切割位点属于A-X-A类型,与其他已经报道的植物病原真菌、细菌以及卵菌中分泌蛋白信号肽切割位点一致。通过上述生物信息学分析方法有效地实现了里氏木霉分泌蛋白的预测,也证明了分泌蛋白的信号肽切割位点具有物种保守性特点。
Trichoderma reesei QM6 awas an important industrial production strains for cellulase production.To identify the secreted protein from T.reesei and clear its characteristic,9 143 protein sequences in T.reesei was analyzed to predict the secreted protein using 5programs including SignalP,ProtComp,TMHMM,big-PI Fungal predictor and TargetP.Meanwhile,the distribution of amino acids,the length of signal peptide as well as the signal peptide cleavage site of secreted protein were analyzed.A total of 356 secrete proteins were found in T.reesei,and the length of amino acids and the signal peptide were different from the plant pathogens.The signal peptide cleavage site belonged to AXA type,which was consistent with other plant pathogenic fungi,bacteria and oomycete.Through the above bioinformatics analysis,the prediction of secreted proteins can be effectively achieved in T.reesei,and the type of signal peptide cleavage site was consistent with other secreted proteins from different species.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2017年第2期28-32,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31560211)
云南省森林灾害预警与控制重点实验室开放基金项目(ZK150004)
云南省教育厅科学研究基金项目(2014Y330)
云南省科技计划项目(2015IA005
2015IA004)
关键词
里氏木霉
分泌蛋白
信号肽
预测软件
Trichoderma reesei
secreted protein
signal peptide
prediction algorithm
