摘要
目的观察丹参对3T3-L1细胞成脂分化和增殖的影响,为丹参应用于自体脂肪移植提供实验依据。方法将含有不同终浓度丹参注射液的完全培养液(0.6 g/L、0.3 g/L、0.15 g/L、0.075 g/L、0.04 g/L、0.02 g/L)分别加入诱导分化的3T3-L1细胞,待细胞分化至成熟脂肪细胞时,利用CCK-8法检测细胞增殖活力;利用油红O染色法、Image-pro plus6.0软件观察脂肪细胞内脂质的聚积;利用甘油三酯GPO-POD法测定脂肪细胞的脂质含量。结果丹参可促进3T3-L1细胞增殖,且呈一定的时间、剂量依赖关系:加药时间点越早,对3T3-L1细胞增殖的促进作用越大;丹参浓度越大,对3T3-L1细胞增殖的促进作用越大。丹参同时也促进了3T3-L1细胞的成脂分化,增加细胞内脂质的聚积,并呈一定的剂量依赖关系。结论丹参能够促进3T3-L1细胞的成脂分化以及增殖,使成熟脂肪细胞的数量增加。
Objective To explore the effect of Salvia mihiorrhiza on proliferation and differentiation of 3T3-L1 cells, so as to provide evidence for further research and clinical application of fat transplantation. Methods The differentiating 3T3-L1 cells after adipogenic induction were incubated with different concentration of S.mihiorrhiza （0.6 g/L 0.3 g/L, 0.15 g/L, 0.075 g/L, 0.04 g/L, 0.02 g/L）. After the 3T3-L1 cells differentiated into mature adipocyte, the cell proliferation viability was determined by CCK-8 assay kit. The lipid droplets accumulation of adipocyte were observed by Oil Red O staining and Image Pro Plus 6.0. The adipogenesis was quantified by measuring lipid content using triglyceride GPO-POD assay kit. Results S. miltiorrhiza promoted a dose- and time-dependent increase in 3T3-L1 cell proliferation viability. The earlier the dosing time was, the better the cell proliferation viability would be; The higher the concentration was, the better the cell proliferation viability would be. Within the six S.mihiorrhiza concentrations confine, S.mihiorrhiza also promoted a dose-dependent increase in 3T3-L1 cell differentiation ability, enhanced the lipid droplets accumulation of 3T3-L1 adipocyte. Conclusion S.mihiorrhiza can promote the increase of adipocyte number via the proliferation and differentiation of 3T3-L1 cells.
作者
林怀安
余力
王健
张波
郑丹宁
周佳
LIN Huaian YU Li WANG Jian ZHANG Bo ZHENG Danning ZHOU Jia.(Department of Plastic and Reconstructive Surgery, Shanghai Ninth People＇s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China)
出处
《组织工程与重建外科杂志》
2017年第1期17-20,共4页
Journal of Tissue Engineering and Reconstructive Surgery
关键词
丹参
3T3-L1
细胞增殖
细胞分化
Salvia mihiorrhiza
3T3-L1
Cell proliferation
Cell differentiation
