摘要
目的探讨夏枯草对不同病理类型的人甲状腺癌细胞增殖的影响,筛选对夏枯草作用较敏感的甲状腺癌细胞株。方法采用MTT比色法测定2.00、5.00、10.00、20.00、40.00、80.00、160.00、200.00 mg/m L夏枯草作用24 h对人正常甲状腺滤泡上皮细胞(HUM-CELL 0097)、人甲状腺乳头状癌细胞(K1)、人甲状腺髓样癌细胞(TT),5.42、10.84、21.75、43.44、86.88、173.75 mg/m L夏枯草作用24 h对人甲状腺滤泡状癌细胞(FTC-133),以及1.56、3.13、6.25、12.00、25.00、50.00、100.00、200.00 mg/m L夏枯草作用24 h对人甲状腺鳞癌细胞(SW579)增殖的影响。结果夏枯草作用24 h对HUM-CELL 0097细胞、K1细胞、TT细胞、FTC-133细胞、SW579细胞的半数抑制浓度(IC50)分别为12.91、2.43、3.44、6.95、31.15 mg/m L。最高抑制浓度为分别为20.00、80.00、40.00、86.88、100.00 mg/m L。结论夏枯草对不同病理类型的人甲状腺癌细胞均有不同程度的抑制增殖作用,在本实验研究的5种细胞株中,K1细胞对夏枯草作用最敏感。
Objective To investigate the effect of Prunella vulgaris L. on the proliferation of different pathological types of thyroid cancer cells, and to screen the most sensitive thyroid cancer cell lines to Prunella vulgaris L. Methods MTT colorimetrie assay was used to determine the effects of Prunella vulgaris L. at levels of 2.00, 5.00, 10.00, 20.00, 40.00, 80.00, 160.00, and 200.00 mg/mL for 24 h on proliferation of human normal thyroid follicular epithelial cells（ HUM-CELL 0097 ）, papillary thyroid cancer cell（K1 ）, and medullary thyroid cancer cell（TT） , 5.42, 10.84, 21.75, 43.44, 86.88, and 173.75 mg/mL for follicular thyroid carcinoma cells （FTC-133）, and 1.56, 3.13, 6.25, 12.00, 25.00, 50. 00, 100.00, and 200.00 mg/mL for thyroid squamous cell carcinoma cells （SW579）, separately. Results The half maximal inhibitory concentration （IC50） of Prunella vulgaris L. to HUM-CELL 0097, K1, TT, FTC - 133,and SW579 for 24 h were 12.91,2.43,3.44, 6.95, and 31.15 mg/mL, respectively. The highest inhibitory concentrations were 20.00,80.00, 40.00, 86.88, and 100.00 mg/mL respectively. Conclusion Prunella vulgaris L. has different degrees of inhibiting proliferation to different pathological types of thyroid cancer cells. The papillary thyroid cancer cell K1 was the most sensitive to Prunella vulgaris L. among the five cell lines.
作者
熊燚
杨哲
赵敏
谭剑斌
杨杏芬
XIONG Yi YANG Zhe ZHAO Min TAN Jian-bin YANG Xing-fen(Medical College of Jinan University, Guangzhou 510632, China School of Public Health, Sun Yat-sen University Guangdong Provincial Center for Disease Control and Prevention)
出处
《华南预防医学》
2017年第1期34-38,共5页
South China Journal of Preventive Medicine
基金
国家科技部"863"项目(2010AA023001)
广东省自然科学基金项目(S2013010013289)
广东省科技计划项目(2013B010404033)
广州市科技计划项目(201300000161)
广东省中药局课题(20132108
20121275)
关键词
夏枯草属
甲状腺癌
细胞增殖
Prunella
Thyroid neoplasms
Cell proliferation
