摘要
目的 研究糖尿病大鼠肾脏蛋白激酶(PK)Cα亚型蛋白表达和mRNA水平的变化。方法 SD大鼠随机分为正常对照组和糖尿病组,成模5周后分别采用PAS染色、免疫组化、RT-PCR、Western blot方法检测单个肾小球面积,肾皮质PKCα的定位和定量以及PKCα mRNA的水平。结果 同正常组相比,糖尿病组大鼠单个肾小球面积增加,但细胞数并无明显变化;肾皮质PKCα的表达明显增强,并且有自胞浆向胞膜的转位;而肾皮质中PKCα mRNA的表达也增加了1.67倍。结论 早期糖尿病大鼠肾脏中PKCα亚型表达和转录水平上调,提示PKCα可能与糖尿病肾病的发生发展有关。
Objective To study the expression and transcription of PKCα in renal tissue of streptozotocin-induced diabetic rats. Methods Hyperglycemia was induced with streptozotocin(55 mg/kg) in Sprague-Dawley rats. After 5 weeks, the expression of PKCα protein and mRNA was measured by immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Results In diabetic animals, the expression of PKCα was greatly enhanced especially in the proximal tubules and the glomeruler mesangial areas with upregulating of the membrane-associated PKCα. RT-PCR analysis showed that mRNA level of PKCα increased by 1.67 folds in diabetic rats as compared with the normal ones. Conclusion The expression of PKCα protein and mRNA is upregulated in early diabetic nephropathy, which suggests an interaction between PKCα and the pathogenesis of diabetic nephropathy.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2002年第4期291-294,共4页
Chinese Journal of Nephrology
