摘要
目的 :在大鼠实验性脑损伤 (TBI)模型中使用不同方案的一氧化氮合酶抑制剂 (NOSI) ,研究其在创伤性脑损伤中合理应用方案。方法 :大鼠 TBI后采用不同的方案应用 NOSI,伤后 1周内对各组大鼠进行生物学行为检测 ,并于伤后第 7天处死取脑 ,观察并测量伤侧大脑皮层坏死区 ,海马 CA2区锥体细胞层面积。结果 :大剂量 N-硝基- L-精氨酸 (L- NNA)大鼠伤后恢复不明显 ,坏死区面积明显扩大 ,伤侧海马 CA2区锥体细胞层面积减少。小剂量L - NNA可促进大鼠伤后神经功能恢复 ,海马 CA2区面积明显增大。结论 :过早大剂量使用 NOSI可加重创伤性脑损伤后的脑继发性损伤。小剂量非选择性抑制剂可明显改善伤后大鼠的神经功能 ,减少海马神经细胞的继发性坏死。
Objective: To observe the effect of the nitric oxide synthase inhibitators (NOSI) on the neural behavior, injured cortex and hippocampus of SD rats after traumatic brain injury (TBI) by administrating different protocols of NOSI, and to explore reasonable way of NOSI use after TBI.Methods: The rats suffered TBI were adminsitrated by L-NNA differently and to evaluate the recovery of the neurological function in a week after TBI. The brains of rats were removed in the seventh day after TBI to explore the changes of injured cortex, hippocampus and measure the area of necrosis in cortex and the area of hippocampal CA2.Results:The values of neurological function test in high dose L-NNA group were significant lower than that in controlled group, while the low dose L-NNA group show the significant higher values. The necrosis area of cortex in the high dose L-NNA group significantly increased.The hippocampal CA2 area in the high dose L-NNA group was significantly attenuated, while that of the low dose L-NNA group was significantly increased. Conclusion: The results of this study suggests that overdose of NOSI used in the early period after TBI would exaggerate the secondary damage. The proper use of low dose L-NNA was useful to the recovery of the neurological function after TBI and reduced the cell's death in the hippcampus. [
出处
《南通医学院学报》
2002年第3期254-256,259,共4页
ACTA Academiae Medicinae Nantong
基金
交通部科技进步"通达计划"资助项目 (编号 :95 -0 6-0 2 -3 1)
关键词
大鼠
创伤性脑损伤
一氧化氮合酶抑制剂
实验性研究
海马
神经功能
traumatic brain injury
nitric oxide synthase
nitric oxide synthase inhibitator
hippocampus
neurological function
Rats
