摘要
目的:评价青藤碱对2,4,6-三硝基苯磺酸(TNBS)灌肠引起的Th1细胞介导的小鼠实验性结肠炎的治疗作用,并探讨其作用机制。方法:为评价青藤碱对结肠炎的治疗作用,将Balb/c小鼠随机分为乙醇对照组,TNBS模型组,青藤碱低(50mg·kg^(-1))、中(100 mg·kg^(-1))、高(200 mg·kg^(-1))剂量组,每组10只。为观察青藤碱对TNBS结肠炎的治疗作用,第1剂TNBS给药后7 d开始灌胃给予青藤碱,持续给药21 d。第28天处死动物,观察结肠黏膜的损伤程度,测定结肠髓过氧化物酶(MPO)活性,ELISA法分别测定结肠炎症细胞因子(TNF-α、IL^(-1)7和IL-23)蛋白水平。结果:与TNBS模型组比较,青藤碱中、高剂量组的体质量、大体损伤评分及组织学表现均显著改善(P<0.05),MPO酶活性显著降低(P<0.05),结肠黏膜中TNF-α、IL^(-1)7和IL-23的蛋白水平均较TNBS组下降(P<0.05)。结论:青藤碱对TNBS诱导的慢性小鼠结肠炎具有治疗作用,作用机制与青藤碱对Th1炎症细胞因子的抑制有关。
Objective： To evaluate the therapeutic effects of sinomenine on T-helper cell type 1-mediated experimental colitis in- duced by 2,4,6-trinitrobenzene sulfonie acid （TNBS） in mice. Methods： Balb/e mice were divided into five groups： ethanol control group, TNBS model group, and sinomenine treatment groups （50, 100 and 200 mg kg^-1 ） with 10 ones in each. Colitis was induced by colonic instillation of TNBS dissolved in 0.1 ml of 50% ethanol. Seven days after the colonic instillation of TNBS, sinomenine was given by gastric gavage once daily for 21 days. The mice were sacrificed on the 28th day, the injury degree of colonic mucosa was ob- served, the colon myeloperoxidase （MPO） activity was determined, and the levels of inflammatory cytokines （TNF-α, IL-17 and IL- 23 ） were determined by ELISA. Results： Compared with those in TNBS model group, the body mass, gross injury score and histologi- cal findings in sinomenine groups at medium dose and high dose were significantly improved （ P 〈 0.05 ）, the activity of MPO signifi- cantly decreased （P 〈 0.05 ）, and the protein levels of TNF-α, IL-17 and IL-23 in colonic mucosa were lower than those in TNBS group （P 〈 0.05 ）. Conclusion： Sinomenine has notable therapeutic effect on TNBS-induced chronic colitis in mice, and the mecha- nism is related to the inhibition of Thl cytokines by sinomenine.
作者
程虹
蒋巧俐
吴东方
Cheng Hong Jiang Qiaoli Wu Dongfang(Pharmacy Department, Zhongnan Hospital, Wuhan University, Wuhan 430071, China)
出处
《中国药师》
CAS
2017年第3期449-452,共4页
China Pharmacist
基金
湖北省自然科学基金项目(编号:2014CFB743)
关键词
盐酸青藤碱
2
4
6-三硝基苯磺酸
实验性结肠炎
小鼠
慢性
Sinomenine hydrochloride
2,4,6-Trinitrobenzene sulfonie acid
Experimental colitis
Mice
Chronic
