摘要
目的:建立绝经后骨质疏松(PMO)模型,外源性补充雌激素,探讨雌二醇(E_2)的骨保护作用及其对胞质活化T细胞核因子1(NFATc1)表达的影响。方法:12周龄Wistar雌性大鼠随机分为假手术组(Sham组)、去势组(OVX组)、实验组(OVX+E_2组),每组10只。OVX+E__2组给予17β-E_2皮下注射,其余2组给予等量生理盐水。16周后,酶联免疫吸附试验(ELISA)测定血清E_2水平,逆转录聚合酶链反应(RT-PCR)法测定骨组织中NFATc1表达量,微型计算机断层成像(Micro-CT)分析大鼠胫骨平台处骨密度(BMD)、骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数目(Tb.N)。结果:Sham组、OVX+E_2组与OVX组相比,BMD、Tb.Th、Tb.N、BV/TV、血清E_2水平升高,NFATc1水平下降(均P<0.05)。结论:雌激素可能通过抑制NFATc1的表达减缓骨量丢失。
Objective： To investigate the protective effects of exogenous estrogen on bone tissue in apostmenopausal osteoporosis （PMO） rat mode, as well as the effect on the expression of NFATc1. Methods：The 12 weeks Wistar rats were randomly divided into sham operation group, OVX group, and OVX＋E2 group and 10 rats in each group. In the OVX＋E2 group, 17β-E2 was injected subcutaneously and the other two groups were given the same volume of normal saline. After 16 weeks, the serum E2 level was determined by ELISA, the expression of NFATcl in bone tissue were measured by RT-PCR, the bone mineral density （BMD） , the fraction of bone tissues （BV/TV） , the thickness of the trabecular bone （Tb.Th） and number of trabecular bone （Tb.N） were measured using Micro-CT analysis. Results＇The 0VX＋E% group and sham operation group compared with the 0VX group, the levels of BMD, BV/TV, Tb.Th, Tb.N and serum E2 were significantly increased, the level of NFATc1 were significantly decreased （P〈 0.05）. Conclusions：Estrogen may prevent bone loss by decreasing NFATcl expression in OVX rats.
出处
《国际生殖健康／计划生育杂志》
CAS
2017年第2期93-96,共4页
Journal of International Reproductive Health/Family Planning
基金
国家卫生计生委科研基金(WKJ2013-2-024)
杭州市医药卫生科技计划重点项目(2011Z003)
杭州市重大科技创新项目(20142013A58)
浙江省科技计划项目(2014C03044-1)
关键词
雌激素类
骨质疏松
绝经后
动物
实验
胞质活化T细胞核因子1
Estrogens
0steoporosis
postmenopausal
Animals
laboratory
Nuclear factor of activated T - cells cytoplasmic
