摘要
目的:探讨p53,Ki-67及E-钙黏蛋白(E-cadherin)在三阴性乳腺癌(triple negative breast cancer,TNBC)组织中的表达及预后的关系。方法:采用免疫组织化学法检测52例TNBC和52例非三阴性乳腺癌(non-triple-negative breast cancer,NTNBC)组织中p53,Ki-67及E-cadherin表达情况,观察3个指标与TNBC患者临床病理学特征及预后的关系。结果:TNBC组织中p53,Ki-67及E-cadherin的阳性表达率分别为67.3%,80.8%,26.9%;而在NTNBC组织中为44.2%,61.5%,48.1%(均P<0.0 5)。在TNBC组织中,p 5 3表达阳性与肿瘤大小、TNM分期及组织学分级有关(均P<0.0 5);Ki-67表达阳性与TNM分期、淋巴结转移有关(均P<0.05);E-cad her in表达阳性与肿瘤大小、TNM分期、淋巴结转移有关(均P<0.05)。在TNBC患者中,p53,Ki-67及E-cadherin表达阳性者与阴性者总体生存率(overall survival,OS)的差异均有统计学意义(P<0.05)。Cox回归分析多因素显示:淋巴结转移、p53、Ki-67及E-cadherin表达是影响TNBC患者总体生存率的独立预后因素(均P<0.05)。结论:TNBC组织中,p53、Ki-67高表达,其表达阳性者预后差,E-cadherin低表达,其表达阳性者预后良好。联合检测p53、Ki-67及E-cadherin表达可为TNBC患者的治疗提供新靶点。
Objective： To investigate the expression of p53, Ki-67, E-cadherin and their prognostic value in triple negative breast cancer （TNBC）. Methods： The expression of p53, Ki-67, E-cadherin was assessed by immunohistochemistry in 52 cases of TNBC and 52 cases of non-triple negative breast cancer （NTNBC）. The association of the clinicopathological variables and the prognostic value of p53, Ki-67 and E-cadherin expression were evaluated in TNBC. Results： The positive expression rates of p53, Ki-67, E-cadherin in TNBC were 67.3%, 80.8%, 26.9%, while the positive expression rates of those in NTNBC were 44.2%, 61.5%, 48.1% （all P〈0.05）. In TNBC, p53 expression was associated with tumor size, TNM stage and histological grade （all P〈0.05）, Ki-67 expression was associated with TNM stage and lymph node metastasis （both P〈0.05）, and E-cadherin expression was correlated with tumor size, TNM stage, and lymph node metastasis （all P〈0.05）. In TNBC, there was significant difference in overall survival （OS） of the positive and negative p53, Ki-67 and E-cadherin patients （all P〈0.05）. Cox regression was used for analysis of factors for TNBC patients. Expression of lymph node metastasis, p53, Ki-67, E-cadherin are independent prognostic factor affecting overall survival of TNBC according to Cox regression analysis of multiple factors （all P〈0.05）. Conclusion： In TNBC, the high expression of p53, Ki-67 has poor prognosis, while the low expression of E-cadherin has good prognosis. Therefore, our data suggests that the combined examination of p53, Ki-67 and E-cadherin expression might be useful prognostic markers in TNBC and might provide molecular targeting therapy of TNBC treatment.
作者
雷珍
钟锡明
周岐
范钰
LEI Zhen ZHONG Ximing ZHOU Qi FAN Yu(Department of Oncology, Affiliated People＇s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002, China)
出处
《临床与病理杂志》
2017年第2期308-314,共7页
Journal of Clinical and Pathological Research
基金
江苏省镇江市社会发展项目(SH2015064)~~
