摘要
目的探讨低聚半乳糖(GOS)对早产大鼠肠道菌群的调节作用。方法早产新生大鼠分为GOS组和对照组,GOS组每天滴管喂养GOS 4g/kg,共14d。于喂养GOS后7d和14d,观察大鼠一般情况并称质量,同时取大鼠直肠末端粪便,行双歧杆菌、乳酸杆菌、肠杆菌及肠球菌培养并行菌落计数。结果 GOS组与对照组在给药期间均正常生长,精神状态等未见异常。与对照组比较,GOS组体质量增长差异无统计学意义(P>0.05),使用GOS 7d时,双歧杆菌、乳酸杆菌、肠杆菌及肠球菌与同时间点对照组比较,差异无统计学意义(P>0.05)。使用GOS 14d后,大鼠粪便中双歧杆菌、乳酸杆菌较对照组明显增加,而肠杆菌、肠球菌明显减少,差异均有统计学意义(P<0.05)。结论低聚半乳糖可促进早产鼠双歧杆菌和乳酸杆菌增长,抑制肠杆菌及肠球菌生长,从而调节肠道菌群平衡。
Objective To explore the effects of galactooligosaccharide on the intestinal flora in premature rats.MethodsPremature Sprague-Dawley rats were randomly divided into GOS group and control group.Premature rats in GOS group were feeding with GOS according 4g/kg by dropper for 14 d.At 7d and 14 d after feeding,the mental state and weight of rats were observed and recorded,the fresh feces in terminal rectum were collected to culture and count the number of colonies of bifidobacteria,lactobacillus,enterobacteria and enterococcus.Results Rats in GOS and the control group were normal growth during the administration period,mental state was not abnormal.Compared with the same time point of the control group,there was no significant difference in weight gain between the GOS group（P〉0.05）.After feeding GOS for 7d,there was no statistically significant difference between the number of colonies of bifidobacteria,lactobacillus,enterobacteria and enterococcus（P〉0.05）.After feeding GOS for 14 d,the number of colonies of bifidobacteria and lactobacillus increased significantly（P〈0.05）,while the number of enterobacteria and enterococcus decreased significantly（P 〈0.05）.Conclusion Galactooligosaccharide could promote the proliferation of bifidobacterium and lactobacillus,inhibit the growth and reproduction of enterobacteria and enterococcus,so regulate the balance of intestinal flora.
出处
《重庆医学》
CAS
北大核心
2017年第8期1015-1016,1019,共3页
Chongqing medicine
基金
江苏省临床医学专项基金(BL2012058)
江苏大学临床医学专项基金(JDLCZX004)
关键词
低聚半乳糖
早产大鼠
肠道菌群
galacto oligosaccharide
preterm rats
intestinal flora
