摘要
从受污染农田中分离到一株扑草净降解菌FR ,经鉴定属于假单胞菌 ,考察了温度及pH对其降解能力的影响 ,确定pH 7.0 ,32℃为最适作用条件 ;设计引物 ,通过PCR扩增得到一条长度约为 5 0 0bp的降解基因保守片段 ,通过对该片段部分序列测定及同源性搜索 ,证实该片段与atzA、triA等均三嗪化合物水解酶基因具有很高的同源性。
A degrading-prometryn strain, isolated from soils exposed to repeated spills of the herbicide, belongs to Pseudomonas. It was proved that the degrading effect was affected by temperature and pH, and that the optimum condition was pH 7.0,32℃. After designing the primers, a 500bp-length degrading-gene conservative sequence was obtained by PCR amplification. With partial sequencing and homology analysis, the result showed there is a high homology between this sequence and the genes of s-triazine hydrolases such as atzA, triA.
出处
《城市环境与城市生态》
CAS
CSSCI
CSCD
北大核心
2002年第4期61-63,共3页
Urban Environment & Urban Ecology
基金
天津市重点自然科学基金资助课题 (99380 2 91)
