摘要
目的对斑马鱼进行几种病原菌的流行病学调查,为后续相关标准的制定提供依据。方法基于细菌的形态学及生理生化特性为基础的传统方法,并与分子生物学方法相结合,最后分离株人工感染健康斑马鱼,观察实验组发病、死亡情况以判定其对斑马鱼的致病性强弱,从而了解影响斑马鱼健康的主要病原菌。结果在所有采样单位共检出3种病原菌,包括嗜水气单胞菌、温和气单胞菌和铜绿假单胞菌。嗜水气单胞菌普遍存在于大多数被检单位,且有较强的致病力。个别单位检出温和气单胞菌和铜绿假单胞菌。温和气单胞菌致病力稍弱;铜绿假单胞菌致病力最弱,感染斑马鱼无典型临床症状,无死亡。结论嗜水气单胞菌可能造成实验用鱼设施的污染,嗜水气单胞菌已证明可以感染人类,此类潜在的人-鱼感染途径应引起重视。由此建议,嗜水气单胞菌应成为重点监控的病原菌。
Objective To conduct an epidemiological investigation of several pathogens in zebrafish,and to provide the basis for subsequent development of relevant standards. Methods This study mainly used traditional methods based on morphological, physiological and biochemical characteristics of bacteria, combined with molecular biology methods,to isolate bacteria and artificially infect healthy zebrafish with the bacteria. Through investigating the incidence and mortality in the experimental group,we distinguished the virulent effect of the pathogens,and further understanding the pathogens affecting the health of zebrafish. Results There were three kinds of pathogens identified in all sampling institutions,including pathogenic Aeromonas hydrophila, Aeromonas sobria and Pseudomonas aeruginosa. Aeromonas hydrophila was detected in most sampling institutions,and showed to possess strong virulence. Aeromonas sobria and Pseudomonas aeruginosa were detected in some sampling institutions,and the virulence of Aeromonas sobria was revealed to be weaker than that of Aeromonas hydrophila. The virulence of Pseudomonas aeruginosa was the weakest among these three bacteria,did not induce typical clinical symptoms and death in the zebrafish. Conclusions Aeromonas hydrophila may cause contamination of fish experimental facilities,and it has been shown to be potentially able to infect humans. Thus regarding the fish infection routes,enough attention should be payed to Aeromonas hydrophila,and it should be the focus in the pathogens monitoring.
作者
冯丽萍
杨迟
林金杏
胡建华
FENG Li-ping YANG Chi LIN Jin-xing HU Jian-hua(Shanghai Laboratory Animal Research Center, Shanghai 201203, China)
出处
《中国比较医学杂志》
CAS
北大核心
2017年第2期25-28,80,共5页
Chinese Journal of Comparative Medicine
基金
国家科技支撑计划课题(No.2013BAK11B02)
上海市科技发展基金(No.15140901000)资助
关键词
斑马鱼
病原体检测
PCR鉴定
人工感染
Zebrafish
Pathogen detection
PCR identification
Artificial infection
