摘要
目的探讨复方钩藤降压片对自发性高血压大鼠心脏重构的保护作用及机制。方法将24只14周龄雄性SHR大鼠随机分为SHR空白组、复方钩藤降压片组、替米沙坦组,另纳入8只同周龄雄性WKY大鼠为正常对照组,干预持续12周。采用无创尾动脉法观察复方钩藤降压片对SHR的降压作用;放免分析法测定血浆白介素-1β(IL-1β)、白介素-10(IL-10)含量;Masson染色观察心脏组织的形态学变化;免疫组化法检测心肌细胞IL-1β、IL-10蛋白表达变化;Western blot法检测心肌细胞IL-1β、IL-10蛋白表达水平。结果与SHR空白组比较,复方钩藤降压片可明显降低血压(P<0.01);与SHR空白组比较,替米沙坦组及复方钩藤降压片组大鼠IL-1β降低,IL-10升高(P<0.01);与替米沙坦组比较,复方钩藤降压片组大鼠IL-1β降低(P<0.05);与SHR空白组相比,复方钩藤降压片组、替米沙坦组心肌间质胶原增生明显减轻;各组大鼠心肌组织蛋白表达体视学图像积分光密度分析,复方钩藤降压片组IL-1β低于替米沙坦组(P<0.05);WesternBIot法检测心肌细胞蛋白表达水平,IL-1β半定量检测结果比较,复方钩藤降压片组、替米沙坦组均低于SHR空白组(P<0.01);复方钩藤降压片组低于替米沙坦组(P<0.05);IL-10半定量检测结果比较,复方钩藤降压片组、替米沙坦组均高于SHR空白组(P<0.05)。结论复方钩藤降压片对SHR心脏重构具有保护作用,其干预机制可能与降低SHR血浆与心脏组织中IL-1β含量,增高IL-10含量有关。
Objective： To investigate the influence of the Fufang Gouteng Tablet on inflammatory factors induced cardiac remodeling in spontaneously hypertensive rats. Methods： 24 male SHR were randomly divided into the SHR blank group, the Fufang Gouteng Tablet group,the telmisartan group. Meanwhile, 8 WKY male rats were in the normal control group. After drugs for 12 weeks,the influence of the Fufang Gouteng Tablet on SHR was measured by noninvasive caudal artery method. The blood pressure,the levels of interleukin-1β（IL-1β） and inter- leukin-10（IL-10） in blood were measured by radioimmunoassay. The SHR heart morphological changes were observed by Masson dyeing. The methods of immunohistochemistry and Western-Blot were used to detect the ex- pression level of IL-1 beta and IL-10 in cardiac muscle cells. Results： Compared with the SHR blank group,the Fufang Gouteng Tablet group could significantly reduce blood pressure （P〈 0.01 ）. Compared with the SHR blank group,the Fufang Gouteng Tablet group and the telmisartan group＇s plasma inflammatory factors IL-1β content were reduced and the inflammatory factors IL-10 were increased（P〈 0.01 ）. Compared with the telmisartan group, the IL-1β in the Fufang Gouteng Tablet group was reduced （P〈0.05）. Compared with the SHR blank group,the myocardial interstitial collagen hyperplasias of the Fufang Gouteng Tablet group and the telmisartan group were significantly reduced. According to the image analysis of integral optical density of stereological protein expression in myocardial tissue of rats,the IL-1β in the Fufang Gouteng Tablet group was lower than that in the telmisartan group （P〈 0.05）. According to the Myocardial cell protein expression level by Western bIot method. The compari- son of semi quantitative detection results indicated the IL-113 of the Fufang Gouteng Tablet group and the telmisartan group was lower than the SHR group （P 〈 0.01 ）. The Fufang Gouteng Tablet group was lower than the telmisartan group on the level of IL-1β（P〈0.05）. The IL-10 of the Fufang Gouteng Tablet group and the telmisartan group was higher than that of the SHR group （P〈 0.05）. Conclusion： The SHR cardiac remodeling can be improved by the Fufang Gouteng Tablet,the protect mechanism may be related to reducing SHR plasma IL-1β content, increasing IL- 10 content.
作者
陈偶英
谭元生
何诗雯
简维雄
张稳
曾勇
杨玉佩
CHEN Ouying TAN Yuansheng HE Shiwen et al(Hunan University of Traditional Chinese Medicine ,Hunan, Changsha 410208, China)
出处
《中国中医急症》
2017年第3期377-380,424,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金项目(81473616)
湖南省科学技术厅科技计划重点项目(2013SK2025)
关键词
复方钩藤降压片
炎症因子
自发性高血压
心脏重构
Fufang Gouteng Tablet
Inflammatory factors
Spontaneous hypertensive rats
Cardiac remodeling
