摘要
目的观察水飞蓟宾(SIL)对心肌缺血再灌注损伤大鼠心肌组织的保护作用并初探其可能的机制。方法取100只实验用大鼠随机分为假手术组,模型组和SIL低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量预处理组(n=20),术前7 d开始灌胃给药;采用结扎冠状动脉30 min的方法建立大鼠心肌缺血再灌注损伤模型;再灌注6 h后,通过高分辨率超声影像系统检测舒张末期左室内径(LVIDd)和收缩末期左室内径(LVIDs)、短轴缩短率(FS)、射血分数(EF)、每搏输出量(SV);计算心脏/体质量比并采用TTC染色法计算心肌梗死面积,生化分析法测定血清中心肌酶含量,HE染色法观察心肌组织形态结构改变,TUNEL法观察心肌细胞凋亡状况,比色法测定心肌组织中抗氧化酶活性和丙二醛(MDA)含量。结果与模型组比较,发现经SIL中、高剂量预处理能够显著降低急性心肌梗死大鼠LVIDd并显著提高FS、EF和SV,其中SIL高剂量预处理组LVIDs显著降低;显著降低心脏/体质量比和心肌组织梗死面积;显著降低血清中心肌酶(AST、CPK、LDH)含量;明显改善心肌组织病变和心肌组织细胞凋亡状况,显著降低心肌细胞凋亡指数(AI),显著提高抗氧化酶(SOD、CAT)活性并显著降低MDA含量,差异均具有统计学意义(P<0.05或P<0.01)。结论 SIL具有改善心功能、减轻心脏水肿、改善心肌组织病变、抑制心肌细胞凋亡、降低氧化应激损伤,提示SIL对心肌缺血再灌注损伤具有一定的保护作用。
Objective： To investigate the effects of Silibinin （SIL） on the myocardial ischemia-reperfusion injury. Methods： 100 rat models were randomly divided into sham operation group,model control group and SIL low -dose [ 100 mg/（ kg. d ） ], medium-dose [ 200 mg/（kg· d ）], high -dose [ 400 mg/（kg· d ） ] pre-treated group （n = 20）. The drugs were given by intragastric administration before operation;the rat models with acute myocardial infarction were made by clamping the artery. 6h after reperfusion, the LVIDd, LVIDs, FS, EF, SV were detected by high frequency ultrasound imaging system ; the ratio of heart weight/body weight was detected, the areas of myocardial infarction was analyzed by TI＇C staining, the content of enzymes in serum were determined, the histopathological changes were observed by HE staining,the eardiomyocyte apoptosis was observed by TUNEL,the activity of antioxidase and the content of MDA in myocardial tissue were determined by colorimetry. Results： Compared with model control group,the LVIDd,LVIDs in SIL medium- and high-dose per-treated groups were significantly de- creased, the FS, EF, SV were significantly increased;the ratio of heart weight/body weight and the myocardial in- farction areas were significantly decreased, the content of AST, CPK, LDH in serum were significantly decreased, the physiological changes and the myocardial eardiomyocyte apoptosis were significantly improved,the AI were significantly decreased;the activity of SOD, CAT in myocardial tissue were significantly increased and the content of MDA was significantly decreased, all of the difference above were statistically significant （P〈 0.05 or P〈 0.01 ）. Conclusion： SIL can effectively improve the cardiac function,lower the cardiac edema,improve histopathological changes and cardiomyocyte apoptosis, and depress oxidative stress,which suggest that SIL has protective effects on the myocardial ischemia-reperfusion injury.
作者
曹旭丹
任春梅
和田田
刘健
CAO Xudan REN Chunmei HE Tiantian et al.(Handan Central Hospital,Hebei,Handan 056001 ,China.)
出处
《中国中医急症》
2017年第3期405-408,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
邯郸市科学技术研究与发展计划项目(1523108145)
关键词
水飞蓟宾
心肌缺血再灌注
心功能
细胞凋亡
氧化应激
Silibinin
Myocardial ischemia-reperfusion
Cardiac function
Apoptosis
Oxidative stress
