摘要
采用绿色环保的碱、尿素、水溶剂体系溶解天然纤维素,利用高压静电法得到再生纤维素微球,通过物理包埋和化学键合两种方式成功对纤维素微球进行了荧光功能化。物理包埋法将纤维素溶液与染料共混,在微球高压静电喷雾成型过程负载罗丹明荧光染料,微球呈现强的橘红色荧光,其最大荧光发射波长与单纯罗丹明水溶液相比发生蓝移;化学键合法以环氧氯丙烷为连接剂在天然纤维素表面引入异硫氰酸荧光素酯(FI-TC),荧光显微镜观察显示微球呈现强的绿色荧光,在水溶剂化作用下,化学键合在微球表面的染料与单纯FITC溶液的荧光发射光谱无明显差别。物理负载方式可实现微球的高染料负载量,为10%(wt),但存在荧光染料的泄漏问题;化学负载方式染料共价键结合,无泄漏问题,但染料负载量较低,约为0.2%(wt)。
Natural cellulose solution dissolved in alkali/urea/water solvent system was used to prepare cellulose microspheres via high-voltage electrospraying technology. Physical encapsulation and covalently linking methods were applied to realize the fluorescent functionalization. Fluorescent cellulose microspheres via physically loading rhodamine B during the process of electrospraying presented strong orange fluorescence. The fluorescence emission peak showed a blue shift compared to that of pure rhodamine B aqueous solution. Fluorescent cellulose microspheres with chemically bonded fluorescein isothiocyanate using epichlorohydrin as coupling agent showed intense green emission under fluorescence microscope. There was no obvious difference between the fluorescence emission spectra of fluorescent cellulose micropsheres and pure dye aqueous solution because of the solvent effect. Physically embedding method possessed the advantage of high dye-loading content, up to 10%（wt）, but also the disadvantage of dye leakage. Chemically linking method could avoid the problem of dye leakage, but its dye loading content was relatively low, about 0.2% （wt）.
作者
宋如愿
李建
管庆顺
吴伟兵
SONG Ru-yuan LI Jian GUAN Qing-shun WU Wei-bing(Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China)
出处
《纤维素科学与技术》
CAS
CSCD
2017年第1期1-6,25,共7页
Journal of Cellulose Science and Technology
基金
国家自然科学基金(31200453)
江苏高校优势学科建设工程资助项目
关键词
荧光微球
荧光探针
物理包埋
化学键合
fluorescent microsphere
fluorescent probe
physical encapsulation
covalently linking
