摘要
【目的】为了探讨碳酸氢钠(NaHCO_3)对嗜水气单胞菌的抑制效果和抑制机理.【方法】研究了不同浓度的NaHCO_3及等摩尔浓度(0.2、0.4、0.8mmol/L)的氯化钠(NaCl)和碳酸氢钾(KHCO_3)对嗜水气单胞菌生长的影响.【结果】随着NaHCO_3浓度的增大,嗜水气单胞菌的生长越来越缓慢.NaHCO_3达到一定浓度后具有杀菌作用,嗜水气单胞菌暴露在0.8、0.4 mmol/L NaHCO_3中达到99.5%死亡率所需要时间分别为1h和4h,暴露在0.2mmol/L NaHCO_3中6h,其死亡率为78.4%.NaHCO_3浓度越高,嗜水气单胞菌致死所需时间越短.暴露12h后,0.4、0.8mmol/L NaHCO_3中均无嗜水气单胞菌存活,0.2 mmol/L NaHCO_3中只有极少数嗜水气单胞菌存活(存活率小于0.01%).0.2、0.4mmol/L的NaHCO_3均能有效抑制嗜水气单胞菌生长,而添加等摩尔浓度的NaCl培养液中,细菌生长不受影响;添加等摩尔浓度KHCO_3的培养液中培养的嗜水气单胞菌,其生长情况与添加NaHCO_3的嗜水气单胞菌相似,生长被明显抑制,结果表明NaHCO_3对嗜水气单胞菌的抑制并非由渗透压引起,而是与碳酸氢根有关.【结论】研究结果为有效控制嗜水气单胞菌病提供了理论依据.
[Objective]In order to research the inhibitory effect and mechanism of Sodium bicarbonate （NaHCO3） on Aeromonas hydrophila. [Method]The effect of different concentration 9f NaHCO3 as well as equimolar sodium chloride（NaCl）and potassium bicarbonate（KHCO3） （0. 2 retool/L, 0.4 mmol/L, 0. 8 mmol/L）on the growth of A. hydrophila was studied. [Result]The higher level of NaHCO3, the slo- wer growth of A. hydrophila. NaHCOa showed bactericidal activity when it reached to a certain concentra- tion. When A. hydrophila was exposed in NaHCO3 of 0.4 mmol/L and 0.8 mmol/L for one hour and four hours respectively,the mortality reached 99.5%. When it was exposed in NaHCOa of 0. 2 mmol/L for six hours.the mortality was only 78.4 % . The lethal time for A. hYdrophila was shortened with the increase of the concentration of NaHCO3. No survived A. hydrophila after being exposed in NaHCO3 Of 0. 4 mmol/ L and 0.8 mmol/L for twelve hours,and very few kept living in 0. 2 mmol/L NaHCO3 （mortality was less than 0.01G）. NaHCO3 of 0.2 mmol L and 0. 4 mmol/L could inhibit the growth of A. hydrophila effec- tively, while it was not affected in equimolar NaCl. Bacteria in equimolar KHCO3 was also inhibited dis-tinctly, showing similar：activity to that in NaHCO3, it sugges;ced that the antimicrobial activity 0f NaHCO3 was due to bicarbonate ion instead of Osmotic pressure. [Conclusion] The result would provide some theo- retical basis for controlling disease caused by A. hydrophila.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2017年第1期32-36,43,共6页
Journal of Gansu Agricultural University
基金
"十二五"国家科技计划项目"池塘优势生物群落调控管理技术研究"(2012BAD25B01)
广东省海洋渔业科技推广专项"内陆池塘复合净化技术示范与推广"(B201400C01)
关键词
嗜水气单胞菌
碳酸氢钠
抑制
生长
Aeromonas hydrophila
sodium bicarbonate
inhibition
growth
