摘要
目的探讨KAI1/CD82基因在鼻咽癌侵袭转移中的作用,分析转染KAI1/CD82基因的内皮祖细胞在鼻咽癌防治中的应用价值。方法应用过表达KAI1/CD82基因的慢病毒转染血管内皮祖细胞,获得高表达KAI1/CD82基因的稳转细胞(KAI1/CD82-EPCs);构建荷人鼻咽癌裸鼠移植瘤模型,通过裸鼠尾静脉注入KAI1/CD82-EPCs;观察KAI1/CD82-EPCs对裸鼠腋下移植瘤及其转移灶的影响。结果 EPCs组、空白组和KAI1/CD82-EPCs组成瘤时间(天)分别为14.70±3.81、15.05±3.85、14.20±3.55,差异无统计学意义(P=0.771);EPCs组、空白组和KAI1/CD82-EPCs组移植瘤的重量(g)分别为1.388±0.204、1.487±0.223、1.485±0.234,差异无统计学意义(P=0.274);EPCs组、空白组和KAI1/CD82-EPCs组肺转移灶生成率分别为55%、45%和10%,差异具有统计学意义(P=0.005),三组肺脏的转移灶数目分别为34.27±5.35、38.44±9.63、17.50±3.54,差异具有统计学意义(P=0.007);注射KAI1/CD82-EPCs的实验组肺转移灶KAI1/CD82呈阳性,EPCs组和空白组肺转移灶KAI1/CD82呈阴性。结论转染KAI1/CD82基因的EPCs对鼻咽癌裸鼠模型的肺转移具有抑制作用。
Purpose To clarify the role of KAI1/CD82 in metastasis of nasopharyngeal carcinom and to evaluate the clinical efficacy of KAI1/CD82-expressing EPCs in the prevention of nasopharyngeal carcinoma. Method Umbilical vein-derived EPCs were infected with KAI1/CD82-expressing lenti-virus to get a KAI1/CD82-overexpressing EPC cell line （KAI1/CD82- EPCs）. A xenograft mouse model of human nasopharyngeal carcinoma was established, and KAI1/CD82-EPCs were injected through the tail vein. The effect of the KAI1/CD82-EPCs on growth and metastasis of the xenograft was observed. Results Time required for tumor formation was 14. 70 ± 3.81, 15.05 ± 3.85, 14. 20 ± 3.55 days respectively for the EPCs, EPCs-NC, and KAI1/CD82-EPCs groups, with no significant difference among the three groups （ P = 0. 771 ）. Weight of the xenograft was （1.388 ±0.204） g, （1.487±0.223） g, （1.485 ±0. 234） g respectively for the EPCs, EPCs-NC, and KAI1/ CD82-EPCs groups, with no significant difference （P = 0. 274）. Rate of lung metastasis was 55% , 45% and 10% for the EPCs, EPCs-NC, and KAI1/CD82-EPC groups, and the difference was significant （ P = 0. 005 ）. Number of metastatic lesions was 34. 27 ±5.35, 38. 44 ± 9.63, 17.50 ± 3.54 for the three groups, and the difference was also significant （ P = 0. 007 ）. Immunohistoehemistry indicated positive KAI1/CD82 expression in metastatic lesion of the KAI1/CD82-EPCs group, but no KAI1/CD82 expression in the EPCs group or EPCs-NC group. Conclusion KAI1/CD82-expressing EPCs inhibits lung metastasis of the xenograft mouse model of human nasopharyngeal car- cinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2017年第3期287-291,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
安徽省高等学校自然科学研究(KJ2015B091by)
蚌埠医学院院级科研课题(BY0925)
