摘要
采用柠檬酸钠还原法制备了粒径约为12nm的纳米金颗粒,并用其标记视黄醇结合蛋白抗体(anti-RBP).在pH=5.29的KH_2PO_4-Na_2HPO_4缓冲溶液中,纳米金标记anti-RBP可与视黄醇结合蛋白(RBP)发生特异性结合使纳米金颗粒凝集成网格结构,由于纳米金颗粒的表面等离子体共振效应,使其在620nm处共振光散射信号显著增强.C_(RBP)在0.1~60ng·mL^(-1)范围内与共振光散射增强值ΔI_(620nm)线性相关,检出限为0.02ng·mL^(-1),用于定量分析人血清中的RBP,方法简单、灵敏,结果满意.
Gold spheres of 12 nm prepared by reduction of sodium citrate are attached to retinol binding protein（anti-RBP）. In pH= 5.29 KH2PO4-Na2HPO4 buffer, the immune reaction between gold-labeled anti-RBP and retinol binding protein（RBP） is carried out, and the resonance light scattering intensity of 620 nm is increased significantly. △I620nm has a good linear relationship with concentration of RBP in 0.01- 60 ng·mL^-1 , and the detection limits of RBP is 0.02 ng·mL^-1. So it is a simple and sensitive method for the quantitative detecting of RBP in the clinical diagnosis with good results.
作者
邹明静
王淑玉
王英玲
李刚
刘雪莹
ZOU Ming-jing WANG Shu-yu WANG Ying-ling LI Gang LIU Xue-ying(Central Laboratory, Heze Medical College, Heze 274030, Shandong, China Clinical Laboratory, Heze City Hospital, Heze 274000, Shandong, China)
出处
《西北师范大学学报（自然科学版）》
CAS
北大核心
2017年第2期70-74,共5页
Journal of Northwest Normal University(Natural Science)
基金
山东省高等学校科技计划资助项目(J11LB01)
菏泽医学专科学校科研资助项目(H14k05)
关键词
纳米金
视黄醇结合蛋白
金标记
等离子体共振光散射
gold nanoparticles
retinol binding protein
gold labeling
plasmon resonance light scattering
