摘要
目的制备中药黄柏提取物双层口腔溃疡膜,并考察其体外释放情况。方法采用流延法制备双层膜,以海藻酸钠浓度(A)、海藻酸钠/氯化钙比例(B)、壳聚糖用量(C)为因素进行正交试验优选保护层处方,采用HPLC法测定盐酸小檗碱含量,并计算体外释放度。结果所制膜性状良好,保护层处方确定A为2.0%,B为1∶2,C为10mL。盐酸小檗碱浓度线性范围为0.2~2.2μg·mL^(-1),平均回收率为100.41%,RSD值为0.85%。双层膜可持续释药6h,3h体外累积释放度83.42%。结论所制膜剂设计合理,工艺简单可行,具有一定应用价值。
OBJECTIVE To prepare double-layer phellodendron oral ulcer membranes,and investigate its release rate in vitrol.METHODS The membranes were prepared using tape-casting technology,the formula of protective layer were optimized by orthogonal experiment with the consistency of sodium alginate（A）,the consistency ratio of sodium alginate to calcium chloride（B） and the amount of chitosan（C） as factors.HPLC was used to measure the contents of berberine and its release rate was also computed.RESULTS The membranes were well shaped and the optimized formula was as follows：A=2.0%,B=1∶2,C=10 mL.The linear ranges of berberine were 0.2-2.2μg·mL-（-1） with an average recovery of 100.41%,RSD 0.85%.A continuous releasing of 6 hours was achieved for the membranes and its cumulative release amount within 3 hours reached 83.42%.CONCLUSION The design of double-layer membrane is reasonable,simple and applicable,the membrane has an application value.
出处
《海峡药学》
2017年第2期15-17,共3页
Strait Pharmaceutical Journal
基金
山东省高校科技计划项目(J14LM56)
山东省教育厅
山东中医药抗病毒协同创新中心项目(XTCX2014B01-07)
关键词
双层膜
黄柏
正交试验
体外释放度
Double-layer membrane
Phellodendron
Orthogonal experiment
Release rate in vitro
