摘要
目的观察参知健脑胶囊单次灌胃对Beagle犬心血管和呼吸系统功能的影响。方法参知健脑胶囊单次灌胃给予Beagle犬的试验设供试品(50、200、800 mg/kg)3个剂量组和1个灭菌注射用水阴性对照组,每组6只动物,雌雄各半。给药容量2.5 mL/kg,给药后连续采集4 h动物血压、心电、呼吸等各项指标的数据,并在检测时间点-5、0(给药结束)、15、30、60、90、120、150、180、240 min对上述各指标数据进行统计学分析和评估。结果供试品各剂量给药组动物的心电图各参数值(心率、P-R间期、QRS间期、QRS电压、Q-T间期和QTc间期)与同一时间点阴性对照组比较,差异均无统计学意义(P>0.05)。未见异常心电图波形,未发现对心脏传导有影响。供试品各剂量给药组动物的收缩压、平均动脉压和舒张压与同一时间点阴性对照组比较,差异无统计学意义(P>0.05)。各剂量给药组动物的呼吸频率和呼吸幅度与阴性对照组比较,差异均无统计学意义(P>0.05)。结论在本试验条件下,参知健脑胶囊单次灌胃给予Beagle犬在50、200、800 mg/kg剂量范围内对动物的呼吸系统和心血管系统均无明显影响。
Objective To observe the effects of single intragastric administration of Shenzhi Jiannao Capsules on the function of cardiovascular and respiratory systems in Beagle dogs. Methods The experiment of single intragastric administration of Shenzhi Jiannao Capsules for Beagle dogs were set up for 3 doses groups （50, 200, 800 mg/kg） of test samples and 1 negative control group of sterile water for injection, with 6 animals in each group, half male and female. The volume of administrated drugs was 2.5 mL/kg, after administration, the related data of blood pressure, electrocar- diogram, breath and others was collected continuously for 4 h, and the data above at the time points of -5, 0 （end of administration）, 15, 30, 60, 90, 120, 150, 180, 240 min was taken statistical analysis and evaluation. Results The val- ues of each electrocardiogram parameter （heart rate, P-R interval, QRS interval, QRS voltage, Q-T interval and QTc in- terval） in each dose group of test samples had no statistically significant differences compared with the negative control group of the same tine points （P 〉 0.05）. There were no abnormal electrocardiogram waveform and no effects on cardiac conduction. The systolic blood pressure, mean arterial pressure, diastolic blood pressure in each dose group of test samples had no statistically significant differences compared with the negative control group of the same time points （P 〉 0.05）. The respiratory frequency and breathing extent in each dose group of test samples had no statistically significant differences compared with the negative control group （P 〉 0.05）. Conclusion Under＇ the condition of this experiment, single intragastric administration of Shenzhi Jiannao Capsules at the doses of 50, 200, 800 mg/kg for Beagle dogs has no obvious effects on the respiratory and cardiovascular systems.
作者
马大勇
蔡舒婷
张斐姝
高强
马克信
韩振蕴
MA Dayong CAI Shuting ZHANG Feishu GAO Qiang MA Kexin HAN Zhenyun(Department of Encephalopathy, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China Graduate College, Beijing University of Chinese Medicine, Beijing 100029, China Department of General Medicine, Eye Hospital, China Academy of Chinese Medical Sciences, Beijing 100040, China)
出处
《中国医药导报》
CAS
2017年第5期4-8,共5页
China Medical Herald
基金
国家十一五科技重大专项"重大新药创制"项目(2009ZX09103-401)
国家自然科学基金资助项目(81673910)
关键词
参知健脑胶囊
BEAGLE犬
单次灌胃
安全药理学
Shenzhi Jiannao Capsules
Beagle dogs
Single intragastric administration
Safety pharmacology
