摘要
目的观察蛇伤胶囊对竹叶青蛇伤血管内皮细胞IL-8、VCAM-1、MIP-1α表达的影响。方法根据课题组前期实验方法制备蛇伤胶囊兔含药血清及复制竹叶青蛇伤细胞模型,设空白组(KB组)、模型组(MX组)和低、中、高浓度蛇伤胶囊药液治疗组(DY组、ZY组、GY组)5组,每组10个样本。在细胞实验中,KB组加入10%正常兔血清培养,MX组在KB组的基础上加入5μg/ml竹叶青蛇毒液培养,DY组、ZY组和GY组在MX组基础上培养6h后分别加入5%、10%和15%的含中药兔血清继续培养。各组分别培养72h后,收集细胞培养液,以酶联免疫吸附法检测细胞培养液中的IL-8、VCAM-1、MIP-1α含量。结果MX组的IL-8、VCAM-1及MIP-1α水平相对KB组均显著升高(均P<0.01),蛇伤胶囊药液治疗组IL-8、VCAM-1及MIP-1α水平相对MX组均不同程度降低,其中ZY组变化有统计学意义(P<0.01或P<0.05)。结论蛇伤胶囊可通过降低IL-8、VCAM-1及MIP-1α表达治疗竹叶青蛇伤所致血管内皮炎症反应。
Objective To observe the influence of Sheshang capsule onvascular endothel ial cells IL-8,VCAM-1, MIP-1 expression in Trimeresurus stejnegeri snakebite. Methods Based on the cell model of sheshang capsules rabbit serum and replication of Trimeresurus stejnegeri prepared b y p revious e xp e r im e n talme thod research. The human umbilical vein endothelial cells （HUVEC） were distributed into the blank group （KB） , the model group （MX） and the low,middle and high dose drug serum group （DY,ZY,GY） after conventional culture and passage and each group had 10 samples. KB was cultured with 10% blank rabbit serum. MX was cultured with 5μg /ml of snake venom in addition. DY,ZY and GY were cultured with the same composition as MX in the first 6 hours and then they were cultured with 5% ,10% and 15% drug serum respectively in addition. After 72 hours culture, the medium was collected to measure the level of IL-8, VCAM-1, and MIP-1 byenzyme linked immunosorbent assay （ELISA）. Results The content of IL-8,VCAM-1 and MIP-1 in MX increased signi f icant ly compared wi th KB （all P 〈0. 01）. 丁he content of IL-8 , VCAM-1 and MIP-1 in DY,ZY and GY all decreased compared wi th MX and ZY decreased significantly（P〈0. 05 or P〈0. 01 ）. Conclusion The Sheshang capsule could t reat the vascular endothelial inflammationin Trimeresurus stejnegeri snakebite by Reducing the expression of IL-8, VCAM-1 and MIP-1.
出处
《蛇志》
2017年第1期6-7,39,共3页
Journal of Snake
基金
国家自然科学基金(81302978)
福建省自然科学基金(2012J01379)
关键词
蛇伤胶囊
竹叶青蛇
血管内皮细胞
炎症反应
Sheshang capsule
Trimeresurus stejnegeri iVascular endothelial cells
Inflammation
