摘要
为明确腊梅硫酯酶基因(Cp FATB)在苜蓿中的表达特点及生理功能,获得苜蓿抗寒育种的基因资源。本实验通过根癌农杆菌介导将Cp FATB转入‘龙牧806’、‘WL319HQ’和‘肇东’3个苜蓿品种中,对其后代进行抗寒性鉴定。结果表明,3个苜蓿品种转Cp FATB基因SP3代(第三代基因修饰材料)种子发芽率、简化活力指数均高于各自对照,其中T‘龙牧806’最高,达到87.79%、208.19;转基因植株叶绿素a、叶绿素b及叶绿素总量显著低于其各自对照苜蓿品种,根系可溶性糖、可溶性蛋白、游离脯氨酸含量显著高于其各自对照苜蓿品种;转Cp FATB基因植株高度和分枝数与其各自对照差异不显著,而越冬率显著高于其各自对照品种,T‘龙牧806’越冬率最高,达到96.60%。转Cp FATB基因促进‘龙牧806’、‘WL319HQ’、‘肇东’苜蓿SP3代种子萌发;抑制苜蓿叶片叶绿素合成。越冬前促进根系可溶性糖、可溶性蛋白、游离脯氨酸含量积累,增强POD、SOD活性,提高了翌年越冬率,说明外源Cp FATB基因有助于苜蓿抗寒性的提高。
The aims are to clarify the expression characteristics and physiological function of Cp FATB（Chimonanthus praecox chloroplast acyl-ACP thioesterase） in alfalfa, and obtain the cold resistance resourcesof alfalfa for breeding. Cp FATB gene was transferred to‘Longmu 806＇,‘WL319HQ＇,‘Zhaodong＇byagrobacterium tumefaciens, and the ability of cold resistance of the third GMOs＇ generation was tested. Theresults showed that the germination rate and simple vigor index of SP3 generation were significantly higherthan that of the control, and that of T‘Longmu 806＇was the highest which achieved 87.79% and 208.19.Chlorophyll a, chlorophyll b and total chlorophyll of transgenic Cp FATB plant were lower than that of thecontrol; the soluble sugar, soluble protein and free proline were significantly higher than that of the control; theplant height and branch number had no significant difference between transgenic Cp FATB plant and thecontrol; the overwinter rate was significantly greater than that of the control, and the overwinter rate of T‘Longma 806＇was the highest which reached 96.60%. Cp FATB gene promoted seed germination of‘Longma806＇,‘WL319HQ＇and‘Zhaodong＇SP3 generation, while inhibited the chlorophyll biosynthesis. It promotedaccumulation of soluble sugar, soluble protein, free proline before overwinter, enhanced the activities of PODand SOD, and then improved the overwinter rate of alfalfa, which indicated that Cp FATB gene improved the ability of cold resistance of alfalfa.
作者
冯鹏
孙力
申晓慧
李如来
郑海燕
李志民
李增杰
姜成
郭伟
Feng Peng Sun Li Shen Xiaohui Li Rulai Zheng Haiyan Li Zhimin Li Zengjie Jiang Cheng Guo Wei(Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences, Jiamusi Heilongjiang 154007 Postdoctoral Workstation of Heilongjiang Academy of Agricultural Sciences, Harbin 150086 Jiamusi University, Jiamusi Heilongjiang 154003)
出处
《中国农学通报》
2017年第9期31-36,共6页
Chinese Agricultural Science Bulletin
基金
中国博士后科学基金"CPFAT基因在紫花苜蓿中的表达特性及提高抗寒性研究"(2013M541423)
关键词
紫花苜蓿
腊梅花硫酯酶基因
生理功能
越冬率
抗寒性
alfalfa
Chimonanthus praecox sulfatase gene
physiological functions
overwinter rate
cold resistance
