摘要
为观察杨梅素对髓鞘损伤精神分裂症样小鼠行为的治疗作用,将40只健康雄性昆明小鼠随机分为正常组、模型组、杨梅素组、氯丙嗪组。正常组给予正常饲料,用混有0.2%(w/w)双环己酮草酰二腙(CPZ)的饲料喂养其他3组小鼠构建脱髓鞘模型。每日用20 mg/kg的杨梅素对杨梅素组小鼠灌胃给药,用4 mg/kg的氯丙嗪对氯丙嗪组小鼠腹腔注射给药。造模给药5周后对小鼠进行各项行为学试验,观察小鼠行为改变。用固蓝(LFB)对髓鞘进行染色并观察各组小鼠髓鞘损伤情况。结果表明:杨梅素组小鼠在行为学试验中各项指标均比模型组有显著改善,对髓鞘损伤小鼠的精神分裂样行为治疗效果与氯丙嗪相当。在固蓝染色中,杨梅素组髓鞘颜色显然比模型组均匀致密。杨梅素对髓鞘损伤小鼠的精神分裂样行为有潜在治疗作用,为其临床应用奠定试验基础。
To study the effect of myricetin on schizophrenia-like behavior of demyelinated mice, forty healthymale Kunming mice were randomly divided into 4 groups： the normal group, the model group, the myricetingroup and the chlorpromazine group. The normal group was fed with normal mouse feed, while the other threegroups were fed with feed that contained 0.2%（w/w） CPZ to construct demyelination. Once a day for 5 weeks,the myricetin group was intragastric administrated with 20 mg/kg myricetin, and the chlorpromazine group wasintraperitoneal injected with 4 mg/kg chlorpromazine. Then changes in the behavior of mice were observed bybehavioral examinations. LFB staining was conducted to display the degree of myelin damage. The results ofbehavioral examinations showed that, compared with the model group, mice in the myricetin group hadsignificantly better behavioral indicators, and myricetin had almost the same therapeutic effect withchlorpromazine. LFB staining showed that the myricetin group had more compact myelin than the model group.In conclusion, myricetin has potential therapeutic effect toward schizophrenia-like behavior of mice caused bydemyelination. This experiment offers a basis for the clinical application of myricetin.
作者
黄景源
张倩颖
丁虹
Huang Jingyuan Zhang Qianying Ding Hong(College of Pharmaceutical Science, Wuhan University, Wuhan 430072 Brandeis University, Waltham, Massachusetts, U.S.A. 02453)
出处
《中国农学通报》
2017年第9期141-145,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金资助项目“Rho激酶作为炎症性肠病防治新靶标的研究”(81273523)
关键词
杨梅素
髓鞘损伤
精神分裂
小鼠
myricetin
demyelination
schizophrenia-like behavior
mouse
