摘要
目的测定新疆不同产地新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量,对药材质量进行初步评价。方法样品经超声提取,点样于高效硅胶G薄层板上,以环己烷-甲苯-乙酸乙酯-甲酸(6∶4∶0.5∶0.4)为展开剂上行展开,在520nm波长进行扫描检测。结果 14个产地新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量为8.215~33.65mg/g,博乐产新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量最高(33.65mg/g),塔什库尔干产新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量最低(8.215mg/g),含量均大于《中国药典》规定。结论采用薄层扫描法测定新疆紫草中β,β’-二甲基丙烯酰阿卡宁含量灵敏度高,准确性好,简便,快速。
Objective To determine the content ofβ,β＇-dimelhylacrylalkannin using TLC scanning method taking preliminary stock of the quality of Arnebia euchromafrom different Areas of Xinjian.MethodsThe samples collected were extracted by the method of ultrasonic extraction and the silica gel G thin layer plate was adopted in the determination with cyclohexane：toluene：ethyl acetate：formic acid（6∶4∶0.5∶0.4）used as developer.The quantitative analysis was conducted by single wavelength scanning at 520 nm.Results The content range ofβ,β＇-dimelhylacrylalkannin in the root of Arnebia euchromafrom 14 areas of Xinjiang is 8.215-33.65 mg/g.in terms of the content of the herb producing bole was highest,up to33.65mg/g.The lowest was Taxkorgan.which was 8.215mg/g.All of them were higher than its requirements in Chinese Pharmacopoeia.Conclusion TLC Scanning Method was shown to be simple,accurate and reproducible in the determination of content ofβ,β＇-dimelhylacrylalkannin in the root of Arnebia euchroma.
作者
徐海燕
丁文欢
张慧
王延蛟
XU Haiyan DING Wenhuan ZHANG Hui WANG Yanjiao(College of TCM Central Laboratory College of Basic Medical, Xinjiang Medical University, Urumqi Xinjiang 830011, China)
出处
《新疆医科大学学报》
CAS
2017年第5期642-645,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金(81360608)
