摘要
目的探讨结核分枝杆菌(Mtb)耐药基因突变位点对异烟肼(INH)体外最小抑菌浓度(MIC)的影响。方法利用基因芯片法筛选耐INH人型Mtb菌株,得到inh A-15(C-T)突变株20株、Kat G315(G-C)突变株46株,另筛出非突变株32株。采用罗氏培养法对菌株进行复苏,并进行梯度浓度INH敏感性试验,了解INH对不同菌株的MIC。结果 INH对inhA-15(C-T)突变菌株的MIC为(0.375±0.079)μg/ml,对Kat G315(G-C)突变菌株的MIC为(5.757±4.060)μg/ml,对无突变菌株的MIC为(0.061±0.050)μg/ml,各组间比较差异有统计学意义(P<0.05)。3株无突变菌株表现为低度耐药,inhA-15(C-T)突变组中有1株表现为敏感,其余表现为耐药,Kat G315(G-C)突变菌株均表现为耐药。结论基因芯片筛选的Mtbinh A-15(C-T)和KatG315(G-C)突变株均对INH存在不同程度的耐药,临床可根据不同突变位点菌株的耐药结果初步判断Mtb对INH的耐药性。
Objective To investigate the effect of drug-resistant gene mutation sites of Mycobacterium tuberculosis（ Mtb） on the minimal inhibitory concentration（MIC） of isoniazid（INH） in vitro.Methods INH-resistant human Mtb strains were screened by Gene chip technology.Then 20 strains with inhA-15（C-T） mutation,and 46 strains with KatG315（G-C） were obtained,and 32 strains without mutation were screened.Bacterial resuscitation was conducted using Lowenstein-Jensen culture.Sensitivity test of INH with gradient concentrations was performed to analyze the MICs of INH for different strains .Results The MIC of INH for the strains with inhA-15 （ C-T） mutation, KatG315（G-C） mutation and non-mutation were（0.375 ＆#177;0.079）μg/ml,（5.757 ＆#177;4.060）μg/ml,and（0.061 ＆#177;0.050）μg/ml,respectively, and there was significant difference among the groups （P＆lt;0.05）.Low drug-resistance existed in 3 strains without mutation.Of strains with inhA-15（C-T） mutation,drug sensitivity existed only in 1 strain and drug resistance in the others .Drug resistance was found in all of the strains with KatG315（G-C） mutation strains.Conclusion The Mtb strains with inhA-15（C-T） or KatG315（G-C） mutation screened by gene chip are resistant to INH in various degrees .In clinical practice ,the resistance of Mtb to INH can be preliminarily determined according to the drug-resistant result of strains with different mutation sites .
作者
马小华
万智敏
向延根
石国民
范任华
彭雪峰
MA Xiao-hua WAN Zhi-min X SHI Guo-min FAN Ren-hua PENG Xue-feng(Department of Clinical Laboratory, Changsha Central Hospital, Changsha 410004 Clinical Medical College, University of South China, Hengyang 421001, Hunan, China)
出处
《广西医学》
CAS
2017年第3期322-325,共4页
Guangxi Medical Journal
基金
湖南省卫生和计划生育委员会科研课题(B2016156)
关键词
结核分枝杆菌
基因突变
异烟肼
最小抑制浓度
基因芯片
Mycobacterium tuberculosis
Gene mutation
Isoniazid
Minimal inhibitory concentration
Gene chip
