摘要
目的:探讨载阿霉素介孔二氧化硅纳米粒(MSNs@DOX)对体外培养的人舌癌Tca-8113细胞增殖及凋亡的作用。方法:透射电镜(TEM)和氮吸附法对介孔二氧化硅纳米粒(MSNs)进行表征;紫外分光光度计检测阿霉素(DOX)释放行为;MTT法检测细胞增殖抑制作用;Hochest33342染色、流式细胞术检测细胞凋亡和周期分布;Western blot检测P53、Bcl-2、Bax和Caspase-3蛋白表达。结果:MSNs形态规则,分布均一,平均粒径、介孔孔径、比表面积和孔容分别为约30nm,19.59682nm、193.4296m2/g、0.947625cm3/g;MSNs细胞相容性良好;与单独DOX相比,载药组的细胞增殖抑制率及凋亡率更显著,且呈一定MSNs浓度依赖性(P<0.05);细胞明显阻滞于G0/G1期;P53、Bax、Caspase-3表达显著升高,Bcl-2表达显著降低。结论:该载药纳米传输系统有望用于舌癌治疗。
Objective： To establish a drug delivery system based on mesoporous silica nanoparticles （MSNs） for its potential role in promoting the anticancer efficacy of doxorubicin （DOX） on Tca-8113 cells. Methods： MSNs were characterized by transmission electron microscope （TEM） and N2 adsorption/desorption method. The drug release profiles of DOX from MSNs were monitored by UV--Vis. The anticancer effect of MSNs@DOX Was demonstrated by MTT assay, Hoechst 33342 staining, and flow cytometry. The related mechanism was explored by Western blot. Results： The particle size of MSNs was about 30 nm and its average pore size, surface area and pore volume were 19. 59682 nm, 193. 4296 m2/g and 0. 947625 cm3/g, respectively. MSNs displayed good cytocompatibility. MSNs@DOX inhibited the proliferation of Tca--8113 cells, induced apoptosis remarkably and arrested cell cycle at G0/G1 checkpoints. Western blot revealed remarkable up--regulated expression of Bax, p53 and caspase-3 and down--regulated expression of Bcl-2. Conclusion： Such a doxorubicin delivery strategy might be promising in tongue cancer therapy.
出处
《口腔医学研究》
CAS
北大核心
2017年第3期249-253,共5页
Journal of Oral Science Research
基金
中央高校自由探索-面上项目(编号:lzujbky-2015-293)
甘肃省卫生行业科研计划管理项目(编号:GWGL2014-06)
