摘要
目的研究血性胸水细胞蜡块制作的效果。方法南京大学附属鼓楼医院病理科2013—2015年临床2 h内送检新鲜血性胸水20例,每份标本均分3份,95%乙醇固定3种不同时间进行细胞蜡块制作,常规切片HE染色观察结果。结果本研究方法制作的细胞蜡块常规HE切片完整、镜下细胞结构清楚、背景干净;三种不同时间做出细胞蜡块个数率并无统计学意义,但从获取细胞量和时间上综合考虑95%乙醇固定4 h的制作效果最好。结论本研究方法选取95%乙醇固定4 h时间段,可得到最佳血性胸水的细胞蜡块制作效果。
Objective To investigate the bloody pleural effusion cell block effect. Method Selecting fresh bloody pleural effusion within 2 hours of 20 cases from 2013 to 2015,comparison the number of cells wax block of three types time of 95% ethanol-fixed by SPSS17. 0,χ~2 test and Hematoxylin Eosin staining. Results The slice is complete,clear cell structure and clean background.Three different time results are not statistically significant,but 95% ethanol-fixed with 4 hours is the best and get more cells. Conclusion The methods of 95% ethanol-fixed time with 4 hours is the best and can apply to make the cell block.
作者
林旭
周强
LIN Xu ZHOU Qiang(Department of Pathology, Drum Tower Hospital Affiliated of Nanjing University, Nanjing, Jiangsu, 210008, China)
出处
《黑龙江医学》
2017年第2期106-107,共2页
Heilongjiang Medical Journal
关键词
血性胸水
细胞蜡块
细胞病理学诊断
Bloody pleural effusion
Cell block
Cytological diagnosis
