摘要
目的明确藏药组方格尔琼体外对抗多房棘球蚴药效学活性及其活性部位。方法将格尔琼经醇提、萃取、凝胶柱层析、硅胶柱层析、液相制备等方法分离纯化得到24个不同部位的化合物,将所得化合物以不同的浓度在体外与原头节共培养,光镜下观察原头节死亡率及结构改变。结果空白组原头节死亡率为13.67±0.577,格尔琼醇提物组为93.67±1.523(P<0.0000),阿苯达唑组为47.67±2.08(P<0.0000),且格尔琼醇提物组原头节死亡后形态与阿苯达唑组有明显差异。继而对格尔琼醇提物进行萃取分离,获得乙酸乙酯部位、正丁醇部位、石油醚部位三个部分,药效学实验发现乙酸乙酯部位组原头节死亡率为93.67±0.577(P<0.0000)。对乙酸乙酯部位进行凝胶柱层析分离,获得Fraction1、Fraction2、Fraction3、Fraction4、Fraction5五个部位,药效学实验发现Fraction3组原头节死亡率为97.33±1.528(P<0.0000)。对Fraction3进行硅胶柱层析分离,获得Fraction3.1、Fraction3.2、Fraction3.3、Fraction3.4、Fraction3.5、Fraction3.6、Fraction3.7、Fraction3.8八个部位,药物实验发现Fraction3.1组原头节死亡率为83.00±4.583(P<0.0000)。对Fraction3.1进行液相制备分离,获得Fraction3.1.1、Fraction3.1.2、Fraction3.1.3、Fraction3.1.4、Fraction3.1.5、Fraction3.1.6、Fraction3.1.7七个部位,药物实验发现Fraction3.1.2组原头节死亡率为83.33±3.06(P<0.0000)。结论格尔琼相比阿苯达唑对体外多房棘球蚴具有较强的杀伤作用,其活性组分为大分子非极性化合物。
Objective To elucidate the pharmacodynamics and active site of Tibetan medicinal herbGeerqiong on the effect of killing protoscolexin vitro.Methods Twenty-six compounds were isolated and purified by alcohol ex- traction, gel column chromatography, silica gel column chromatography and preparative liquid phase.The protoscolex was treated with different concentrations of compounds in vitro. Observe the mortality and structural changes of the protoscolex under light mieroseope.Result The mortality rate of protoscolex in control group was 13.67±0.577 and ethanol extract group was 93.67±1.523 （ P〈0.0000）, ABZ group was 47.67±2.08 （ P〈0.0000）, respectively.The structure changes of protoscolex treated with ABZ and ethanol extract was significantly different.Three parts were ob- tained from the extraction of alcohol extract which named ethyl acetate part, n-butanol part and petroleum etherpart. We found that the mortality rate of protoscolex treated with ethyl acetate part was 93.67±0.577 （P〈0.0000）. Frac- tionl, Fraction2, Fraction3, Fraction4, Fraction5 were obtained from the ethyl acetate site with gel column chroma- tography.The pharmaceutic active experiment found that the mortality rate of protoscolex treated with Fraction3 was 97.33 ± 1.528 （ P 〈 0. 0000）. （ 4 ） Fraction3.1, Fraction3.2, Fraction3.3, Fraction3.4, Fraction3.5, Fraction3.6, Frac- tion3.7,Fraction3.8 were obtained from Fraction3 with Silica gel column chromatography.The pharmaceutic active experiment found that the mortality rate of protoscolex treated with Fraction3.1 was 83.00±4.583 （P〈0.0000）. （5） Fraction3.1.1, Fraction3.1.2, Fraction3.1.3, Fraction3.1.4, Fraction3.1.5, Fraction3.1.6, Fraction3.1.7 were obtained from Fraction3.1 with S liquid phase.The pharmaceutic active experiment found that the mortality rate of protoscolex treated with Fraction3.1 was 83.33 ± 3.06 （ P 〈 0.000）. Conclusion Geerqiong has a stronger effect on protoscolex compared with albendazole in vitroand its active site is macro-molecular non-polar compound.
作者
刘文磊
蒋巧艳
洛桑达哇
杨宝良
樊海宁
邓勇
汤锋
LIU Wen-lei JIANG Qiao-yan LUOSANG Da-wa YANG Bao-liang FAN Hai-ning DENG Yong TANG Feng(Department of Hepatopancreatobiliary Surgery ,Affiliated Hospital of Qinghai University ,Qinghai ,Xining 810001 Research Center for High Altitude Medical Sciences, Qinghai University, Qinghai, Xining 810001 Key Lab. for hydatid diseases of Qinghai Province ,Xining,Qinghai 810001)
出处
《中国高原医学与生物学杂志》
CAS
2017年第1期55-62,共8页
Journal of Chinese High Altitude Medicine & Biology
基金
青海省重大科技专项(2016-SF-A5)
关键词
藏药
体外培养
多房棘球蚴
药效活性部位
Tibetan medicinein In vitro Echinococcosis Multilocularis Pharmacodynamics active fraction
