摘要
目的 探讨白细胞介素（IL）36α对小鼠银屑病样皮损及CC趋化因子配体20（CCL20）的影响。方法 30只BALB/c雌性小鼠分为3组：凡士林空白对照组（对照组）、咪喹莫特模型组（模型组）以及IL-36α实验组（实验组）。用小鼠银屑病皮损面积和疾病严重程度评分（PASI）观察银屑病样小鼠皮损动态变化。光镜下观察皮损组织形态学变化，并测量表皮层厚度。用实时荧光定量PCR、 Western印迹检测对照组与模型组小鼠皮损中IL-36α表达情况，用实时荧光定量PCR、Western 印迹法、免疫组化方法检测小鼠皮损中CCL20的含量变化。结果 模型组皮损中IL-36α mRNA表达水平明显高于对照组（-Ct值分别为0.0195 ± 0.0059、 0.0012 ± 0.0004，两组比较，P 〈 0.05）；模型组皮损中IL-36α蛋白表达水平明显高于对照组（分别为3.922 ± 0.248、0.690 ± 0.025，两组比较，P 〈 0.05）。对照组、实验组CCL20 mRNA表达（-Ct值）分别为0.378 ± 0.075、2.152 ± 0.793，与模型组（0.999 ± 0.178）比较，差异有统计学意义（P 〈 0.05）；对照组、实验组CCL20蛋白表达结果分别为0.025 ± 0.009、0.397 ± 0.033，与模型组（0.145 ± 0.030）比较，差异有统计学意义（P 〈 0.05）。免疫组化结果表明，实验组皮损中CCL20的含量较模型组显著增加，差异有统计学意义（Z = 2.294，P 〈 0.05）。结论 IL-36α可通过促进CCL20表达，加重银屑病样小鼠皮损病变。
Objective To evaluate effects of interleukin-36α （IL-36α） on psoriasiform skin lesions and C-C motif chemokine ligand 20 （CCL20） in mice. Methods Totally, 30 BALB/c female mice were randomly and equally divided into 3 groups： control group treated with topical vaseline cream on the shaved back and intracutaneous injection with phosphate buffer saline （PBS）, model group treated with topical imiquimod cream on the shaved back and intracutaneous injection with PBS, experimental group treated with topical imiquimod cream on the shaved back and intracutaneous injection with IL-36α solution. Psoriasis area severity index （PASI） was used to evaluate changes of psoriasiform skin lesions in mice, and light microscopy to observe morphological changes of skin lesions and to measure the thickness of the epidermis. Real-time fluorescence-based quantitative PCR （qRT-PCR） and Western blot analysis were performed to determine the of IL-36α in skin lesions in the control group and model group, and qRT-PCR, Western blot analysis and immunohistochemical study to evaluate changes of CCL20 levels in skin lesions. Results The model group showed significantly increased mRNA （-Ct value： 0.0195 ± 0.0059） and protein （3.922 ± 0.248） of IL-36α compared with the control group （mRNA： 0.0012 ± 0.0004, P 〈 0.05; protein： 0.690 ± 0.025, P 〈 0.05）. The mRNA and protein of CCL20 were significantly higher in the experimental group than those in the model group （mRNA： 2.152 ± 0.793 vs. 0.999 ± 0.178; protein： 0.397 ± 0.033 vs. 0.145 ± 0.030; both P 〈 0.05）, and higher in the model group than those in the control group （mRNA： 0.378 ± 0.075; protein： 0.025 ± 0.009; both P 〈 0.05）. Immunohistochemical study showed that the intensity of CCL20 in skin lesions significantly increased in the experimental group compared with that in the model group （Z = 2.294, P 〈 0.05）. Conclusion IL-36α may aggravate psoriasiform skin inflammation in mice by promoting CCL20 .
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2017年第4期263-267,共5页
Chinese Journal of Dermatology
基金
广东省中医药局科研课题（20141008）
