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ATAD2对人胶质瘤细胞系转移及上皮间质转换的影响 被引量:1

Effect of ATAD2 on metastasis and epithelial-mesenchymal transition of human glioblastoma cell lines
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摘要 目的探讨三磷酸腺苷酶家族蛋白2(ATAD2)对人胶质瘤细胞的影响。方法用real-time PCR及Western blot检测ATAD2在人胶质瘤细胞系U87MG、U251和正常人星形胶质细胞的表达。U87MG和U251分为对照组(control)、脂质体对照组(mock)、ATAD2 siRNA(转染特异性siRNA)和ATAD2 overexpression(过表达ATAD2)4组,MTT法、Transwell实验观察细胞增殖和侵袭迁移。Western blot检测上皮间质转换标志蛋白上皮钙黏素、神经钙黏素和波形蛋白的表达。结果 U87MG和U251细胞ATAD2高表达。与对照相比,ATAD2 siRNA抑制U87MG和U251细胞增殖、侵袭、迁移、神经钙黏素及波形蛋白表达,促进上皮钙黏素表达(P<0.05);ATAD2 overexpression促进细胞增殖、侵袭、迁移、神经钙黏素和波形蛋白表达,降低上皮钙黏素表达(P<0.05)。结论 ATAD2可能通过调节上皮间充质转换进程参与人胶质瘤细胞转移。 Objective To analyze the effect of ATAD2 on glioma cell lines. Methods ATAD2 level in U87 MG,U251 and normal human astrocytes was detected by RT-PCR and Western blot. U87 MG and U251 cells were divided into four groups: control,mock( lipofection control),ATAD2 siRNA( transfected with ATAD2 siRNA) and ATAD2 overexpression( transfected with ATAD2). Cell proliferation,migration and invasion were respectively measured by MTT and Transwell assay. The level of E-cadherin,N-cadherin and Vimentin was measured by Western blot. Results ATAD2 was highly expressed in U87 MG and U251 cells. Compared with control,cell proliferation,invasion,migration,N-cadherin and Vimentin expression were decreased by ATAD2 siRNA,while they were promoted by ATAD2 overexpression( P〈0. 05). E-cadherin expression was upregulated by ATAD2 siRNA and it was inhibited by ATAD2 overexpression( P〈0. 05). Conclusions ATAD2 participants in human glioma cells metastasis via epithelial-mesenchymal transition( EMT).
出处 《基础医学与临床》 CSCD 2017年第4期512-517,共6页 Basic and Clinical Medicine
基金 国家博士后科学基金(2014M552708)
关键词 三磷酸腺苷酶家族蛋白2 胶质瘤 侵袭 迁移 上皮间质转换 ATAD2 glioma invasion migration EMT
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