摘要
目的:探索医疗设备维修工具细菌污染情况的监测与消毒方法。方法:以某医院医疗设备维修工具中使用频率最高的螺丝刀、内六角扳手、万用表共30件为研究对象,将其放入医用臭氧消毒柜,臭氧消毒5 min,分别于消毒前后用无菌棉拭子采集维修工具表面的样本,经37℃、48 h培养后观察菌落数,进行统计分析,并对其中的病原菌进行分类鉴定,比较不同种类维修工具的污染情况。结果:消毒前维修工具表面样本菌落数达(132.68±37.02)cfu/cm^2,经臭氧消毒5 min后样本菌落数减少到(6.44±1.08)cfu/cm^2,细菌灭活率为95.15%。消毒前后样本菌落数差异具有统计学意义(P<0.01)。消毒前样本中鉴定出金黄色葡萄球菌、大肠埃希菌等病原菌,消毒后均未检出。3种维修工具表面菌落数差异具有统计学意义,病原菌检出率螺丝刀最高,万用表最低。结论:医疗设备维修工具表面携带较多细菌,包含多种病原菌,具有潜在的医院感染风险,应采取感染控制措施,定期采用臭氧消毒。
Objective To survey bacterium pollution state on the surface of medical equipment maintenance tools,and then observe the disinfection effect of ozone.Methods Totally 30 medical equipment maintenance tools including screwdriver,hexagona wrench and multimeter from certain hospital were placed in ozone disinfection cabinet,and then disinfected by ozone up to 5 minutes.Samples on the surface of tools were collected by cotton swabs before and after disinfection,cultured for 48hours in 37℃,and then the bacterial colony counts were measured and analyzed,and pathogenicity bacteria were identified.The pollution couditions on kinds of tools were compared.Results The surface of the tools was polluted severely,bacterial colony counts of which before disinfection reached（132.68±37.02）cfu/cm^2,and decreased to（6.44±1.08）cfu/cm^2after 5-minutes＇disinfecting.There was statistically significant difference in the bacterial counts before and after the disinfection（P0.01）,and the inactivation ratio was up to 95.15%.Pathogenicity bacteria such as staphylococcus aureus and escherichia coli were detected before the disinfection,and killed after that.The bacteria counts were significantly different in the three kinds of tools,and the screwdriver gained the highest count while the multimeter had the lowest one.Conclusion The surface of medical equipment maintenance tools is polluted by bacteria severely,including some pathogenicity bacteria,and should be disinfected regularly.Ozone can kill bacteria rapidly,and the tools can achieve sanitary standard after 5-minutes′ozone disinfecting.
出处
《医疗卫生装备》
CAS
2017年第3期94-96,共3页
Chinese Medical Equipment Journal
基金
全军医学科技青年培育计划(16QNP058)
济南军区总医院院长基金(2015GL01
2015BS07)
关键词
医疗设备
维修工具
细菌监测
消毒
臭氧
medical equipment
maintenance tool
bacterium monitoring
disinfection
ozone
