摘要
目的评价实验室对血红蛋白组分中血红蛋白A2(Hb A2)和血红蛋白F(Hb F)的检测能力。方法 2012至2016年每年进行2次有关Hb A2和Hb F的室间质量评价,每次评价5个样本;按室间质量评价流程要求,各实验室在规定时间内检测样本并上传检测结果,依据回报结果统计各检测系统5年的使用分布情况、各实验室的合格率,计算并分析各检测系统、各检测方法的离散程度及不同浓度水平质控品检测结果变异情况。结果 2016年,高效液相色谱法(HPLC)和毛细管电泳法(CE)使用率分别提高至46.1%(82/178)、18.0%(32/178);2012至2016年,Hb A2和Hb F合格率由51.5%(34/66)、60.6%(40/66)分别提高至93.3%(166/178)、92.1%(164/178);各检测系统的平均变异系数(CV)逐年缩小;Bio-Rad VariantⅡ和Sebia CAPILLARYS 2检测系统高、中、低值Hb A2质控品检测结果平均CV较好,可控制在6.0%内;HPLC和CE可定量分析Hb A2和Hb F指标,总体检测能力优于琼脂糖凝胶电泳法。结论通过室间质量评价考核实验室检测Hb A2和Hb F能力,量化Hb A2和Hb F指标,为地中海贫血筛查和防治工作提供质量保证与数据支持。
Objective To evaluate the detection competence of Hb A2 and Hb F in Guangxi medical laboratories. Methods The external quality assessment（ EQA） of Hb A2 and Hb F was conducted twice a year and five samples was detected each time during 2012 to2016. The laboratories participated in EQA completed the samples＇ detection and submitted the detection results at specified time according to the requirements of EQA. The distribution of each detection system,the qualification rate of each laboratory,the variation degrees of each detection system and each detection method,and the variations of results for different levels of quality control（ QC） materials during 5 years were analyzed based on the returned results. Results The application of high performance liquid chromatography（ HPLC） and capillary electrophoresis（ CE） increased year by year and their usage rates in 2016 reached up to 46. 1%（ 82/178） and18. 0%（ 32/178）,respectively. The qualification rates of Hb A2 and Hb F increased from 51. 5%（ 34/66） and 60. 6%（ 40/66） in2012 to 93. 3%（ 166/178） and 92. 1%（ 164/178） in 2016,respectively. The average coefficient of variation（ CV） of each detection system decreased year by year. There were good CVs for the results of high,medium and low levels of Hb A2 QC materials detected by the Bio-Rad Variant Ⅱ and Sebia CAPILLARYS 2 systems,and they were less than 6. 0%. HPLC and CE could quantitatively detect the Hb A2 and Hb F levels,and their total detection competence was superior to that of agarose gel electrophoresis. Conclusion EQA can assess the ability of one laboratory detecting Hb A2 and Hb F,and quantitatively analyze the levels of Hb A2 and Hb F,which may provide the quality assurance and data support for the screening and prevention of thalassemia.
出处
《临床检验杂志》
CAS
CSCD
2017年第2期142-144,共3页
Chinese Journal of Clinical Laboratory Science
基金
广西壮族自治区卫计委科研课题(z2014221)
