摘要
四溴双酚A(TBBPA)作为一种被广泛使用的溴代阻燃剂,在环境中广泛存在.从活性污泥中筛选出一株TBBPA的好氧降解菌,经16S rDNA测序鉴定为假单胞菌属,命名为Pseudomonas sp.JDT.该菌在40天内对TBBPA的脱溴率为12.9%.外加碳源能够促进该菌对TBBPA的矿化,且低浓度酵母提取物的促进作用高于葡萄糖和高浓度酵母提取物.利用^(14)C示踪技术,在持续加有外来碳源的纯菌培养体系中测定JDT对TBBPA的降解,发现18.5天后TBBPA的矿化率为2.7%,并检测到两种极性代谢产物,分别占有机可提取部分总放射性量的11.2%和47.2%.Pseudomonas sp.JDT可以作为研究环境中TBBPA微生物降解途径的实验材料.
A TBBPA-degrading aerobic bacterial strain was isolated from an activated sludge and was identified as a member of the genus Pseudomonas according to 16 S rDNA gene analysis.The strain was named as Pseudomonas sp.JDT.After 40-day incubation,12.9% of TBBPA was debrominated by Pseudomonas sp.JDT.Extra carbon addition enhanced TBBPA mineralization by JDT.The inductive effects from yeast extract at a lower concentration was higher than that at a higher concentration.In the presence of extra carbon sources,2.7% of initial TBBPA was mineralized after 18.5days incubation.Two metabolites with a higher polarity than TBBPA were detected at the end of incubation,accounting for 11.2% and 47.2% of extractable radioactivity,respectively.The results suggest that Pseudomonas sp.JDT may be used for study on the biodegradation pathway of TBBPA in the environment.
作者
赵莹莹
王永峰
孙棐斐
郭红岩
季荣
Zhao Yingying Wang Yongfeng Sun Feifei Guo Hongyan Ji Rong(State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment, Nanjing University, Nanjing, 210023, China)
出处
《南京大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第2期218-226,共9页
Journal of Nanjing University（Natural Science）
基金
国家自然科学基金(21237001)
