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检测诺如病毒的罗氏LightMix~ Kit方法与行业标准方法(SN/T 2626—2010)的比较研究

A comparative study of Roche LightMix~ Kit and the industry standard method(SN/T 2626-2010) for the detection of Norovirus
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摘要 目的结合不同标准和规范方法,对罗氏LightMix~Kit诺如病毒检测试剂盒(以下简称罗氏方法)进行评价。方法通过对217份门诊腹泻病人粪便标本和158份市场零售贝类样品中诺如病毒核酸平行检测,对比罗氏方法与我国SN/T 2626—2010《国境口岸诺如病毒检测方法》中逆转录-聚合酶链反应检测诺如病毒核酸方法(以下简称SN/T方法)对粪便标本与贝类样品中诺如病毒核酸检测结果的差异。结果粪便标本的罗氏方法检测阳性率为27.2%(59/217),而SN/T方法检测阳性率为17.5%(38/217),两方法结果一致率为85.7%,检出率差异有统计学意义(P<0.05)。贝类样品的罗氏方法检测阳性率为32.9%(52/158),SN/T方法检测阳性率为30.4%(48/158),两方法对诺如病毒检测结果的一致率为87.3%,检出率差异无统计学意义(P>0.05)。结论市场零售贝类样品中诺如病毒污染率较高,罗氏方法以其快速、简便、灵敏、可靠的优点值得在食品微生物检测行业推广。 Objective To compare LightMix- Kit Norovirus detection kit from Roche diagnostics company( hereinafter referred to as"Roche method") with different methods of standards and norms. Methods Two hundred and twenty-seven stool samples of diarrhea patients and 158 shellfish samples from retail markets were detected by Roche method and SN / T 2626-2010 detection of Norovirus at frontier port reverse transcription polymerase chain reaction to detect Norovirus method( hereinafter referred to as SN / T method). By parallel detection,the differences between the two methods were compared. Results For stool samples,about 27. 2%( 59 /217) stool samples were positive by Roche method,while17. 5%( 38 /217) samples were positive by SN / T method. The consistence between the two methods was 85. 7% and the detection rate of Roche methods was significantly higher than that of SN / T method( P 0. 05). For shellfish samples,about32. 9%( 52 /158) shellfish samples were positives by Roche method and 30. 4%( 48 /158) samples were positive by SN / T method. The consistence between the two methods was 87. 3% and the detection rate had no significant difference( P 0. 05).Conclusion The Norovirus contamination of shellfish samples from retail market was high. Roche method was rapid,simple,sensitive and reliable to detect Norovirus,and was worthy to promote in food microbiology testing industry.
出处 《中国食品卫生杂志》 2017年第1期47-51,共5页 Chinese Journal of Food Hygiene
关键词 诺如病毒 逆转录-聚合酶链反应 粪便 贝类 检测方法 比较 食品安全 Norovirus reverse transcription polymerase chain reaction stool shellfish detection method comparison food safety
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