摘要
目的评估MALDI-TOF MS鉴定酵母菌的能力。方法分别用VITEK MS和API ID 32C系统同时鉴定387株临床分离酵母菌。以ITS测序结果作为比较标准。比较MALDI-TOF MS、API ID 32C结果准确率。结果 VITEK MS鉴定酵母菌准确率为97.7%,API ID 32C鉴定准确率为93.0%,差异有统计学意义(χ~2=9.439,P=0.002);MALDITOF与ITS测序相比,差异无统计学意义。结论 MALDITOF可对临床常见酵母菌快速、准确鉴定到种。
Objective To evaluate the ability of MALDI-TOF MS in identification of the yeast isolated from clinical specimens. Methods 387 strains of yeasts were tested. All the strains were from clinical specimen and identified by VITEK MS and API ID 32 C system at the same time. Sequencing of the internal transcribed spacer（ITS） regions of ribosomal DNA was used as the reference method in the analysis of a total of 387 yeast isolates. The accuracy of detected results was analyzed by the χ~2test. Results The identification accuracy rates of VITEK MS,API ID 32 C were respectively 97. 7% and 93. 0%. The difference was statistically significant（χ~2= 9. 439,P =0. 002）. There was no statistical significance between MALDI-TOF and ITS sequencing. Conclusion MALDITOF is a rapid simple and accurate method which is identified to species level in identification of yeasts.
出处
《安徽医科大学学报》
CAS
北大核心
2017年第4期501-504,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省高校省级自然科学研究项目(编号:KJ2015A337)
