摘要
为获得具有生防潜力的内生细菌,采用常规组织表面消毒法对槟榔进行内生细菌分离,共获得16株内生细菌。经筛选发现,菌株BLG1抑菌谱较广,尤其对水稻纹枯病菌的皿内抑菌率可达95.00%。进一步研究该菌株胞内及胞外粗提物的抑菌活性发现,该菌株活性物质主要分布在胞外正丁醇相中。盆栽试验表明,该菌株发酵液对水稻纹枯病有很好的防效,可达67.05%,与对照药剂10%井冈霉素水剂效果(66.47%)相当。因此,BLG1菌株具有深入研究和开发的价值。
In order to obtain bio-control endophytic bacteria,sixteen endophytic bacteria were isolated from Areca catechu L.by conventional surface disinfection method.The strain BLG1 showed excellent antimicrobial activities,especially,the inhibition rate against Rhizoctonia solani reached 95.00%.Crude extracts from endo-and extra-cell of the strain were tested,and the result showed that the active components mainly were distributed in extracellular part extracted by n-butyl alcohol.Pot experiment test showed that the strain BLG1 had good control effect(67.05%) against Rhizoctonia solani,which was the same as 10% Jinggangmycin aqueous solution(66.47%).Therefore,the strain BLG1 has values of further research and development.
出处
《河南农业科学》
CSCD
北大核心
2017年第2期60-63,共4页
Journal of Henan Agricultural Sciences
基金
海南省自然科学基金项目(314054
311028)
关键词
槟榔
内生细菌
抗菌活性
盆栽试验
水稻纹枯病
防效
Areca catechu L
endophytic bacterium
antimicrobial activity
pot experiment
Rhizoctonia solani
control effect
