摘要
目的:探讨易洛魁家族同源盒基因IRX1(Iroquois homeobox gene)在胶质瘤中的表达及其临床意义。方法:收集4位胶质瘤患者的胶质瘤组织和癌旁/正常组织各1例,收集54例胶质瘤组织(WHO Ⅰ级5例,Ⅱ级16例,Ⅲ级14例,Ⅳ级19例),采用PCR方法检测IRX1基因在胶质瘤细胞(U87、U373、LN229和T98G)中的表达,Western Blot检测4组同一患者来源的胶质瘤组织中IRX1的表达,免疫组织化学法(IHC)检测IRX1在胶质瘤组织中的表达及临床特征。结果:PCR结果表明以正常脑组织为对照,IRX1基因在多种胶质瘤细胞中均有表达(P<0.05);Western Blot结果显示IRX1蛋白在胶质瘤组织中的表达显著高于癌旁组织或正常组织(P<0.05);IHC结果显示IRX1蛋白在良性胶质瘤组织中主要定位于胞浆,而在恶性胶质瘤组织中定位于细胞核,且其表达量与胶质瘤的恶性程度相关,恶性程度越高,其在细胞核中的表达量越高(P<0.05)。结论:IRX1可能参与调控恶性胶质瘤的发生发展过程,IRX1的表达与胶质瘤的恶性程度有关,暗示了IRX1可作为判断胶质瘤预后以及肿瘤靶向分子治疗的指标。
Objective： To investigate the expression and clinical significance of Iroquois homeobox gene IRX1 in human glioma. Methods： 4 groups of glioma tissues and adjacent normal brain tissues form the same patient were collected, respectively. Another 54 glioma tissue samples were prepared too, of which 21 were classified as low-grade [5 pilocytic astrocytomas （WHO I） and 16 diffuse as- trocytomas （WHO II）] and 33 were classified as high-grade gliomas [14 anaplasia astrocytomas （WHO III） and 19 primary glioblastomas （WHO IV）]. The expression oflRX1 gene in glioma cells （U87, U373, LN229 and T98G） was detected by PCR. And the expression of IRX1 protein in glioma tissues was verified by western blot and IHC. Results： IRX1 gene was expressed in U373, LN229 and T98G cells, but no expression in U87 cells and adjacent normal brain tissues. Furthermore, the results of western blot and IHC showed that the ex- pression levels oflRX1 protein was higher in glioma tissues than in normal tissues （P〈0.05）, and a high level oflRX1 expression was significantly more common in glioma tissues of more advanced grade than those of lower grade （P 〈 0.05）. Conclusion： IRX1 might in- volve in the regulation of onset and progress of glioma, of which the expression was related to the grade of carcinoma, indicating that IRX1 might be used as an indicator for evaluation of prognosis and malignancy level as well as targeted molecular therapy of human ma- lignant glioma.
出处
《现代生物医学进展》
CAS
2017年第7期1229-1232,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81272419
81572983)
陕西省社会发展科技攻关项目(2015SF027)
关键词
IRX1基因
胶质瘤
表达
临床意义
IRX1 gene
Human glioma
Expressions
Clinical significance
