摘要
目的探讨在吸烟人群中wnt/β-catenin信号转导途径的激活情况以及其与肺鳞状细胞癌发病率的相关性。方法选取武汉大学人民医院呼吸内科肺鳞状细胞癌并且有吸烟史患者30例（A组）、鳞癌并且无吸烟史患者30例（B组）、支气管扩张有吸烟史患者30例（C组）、支气管扩张症无吸烟史患者30例（D组）。在患者经过外科治疗后，将切除病变组织运用流式细胞仪对CD68＋进行检测，随后运用蛋白质印迹杂交方法对TNF-α、wnt-2、β-catenin和TCF-4的表达情况进行检测，运用RT-PCR法对TNF-α、wnt-2、β-catenin、TCF-4转录mRNA浓度进行检测。结果经过免疫组化研究表明，A、B、C、D组患者细胞中TNF-α、CD68＋的表达分别为0.895±0.036、0.931±0.237，0.125±0.018、0.631±0.064，0.083±0.042、0.311±0.062，0.053±0.004、0.196±0.020，组间两两比较差异均有统计学意义（P均〈0.05）。western blot检测结果表明wnt-2、β-catenin、TCF-4的表达水平A、B、C、D组呈逐渐递减趋势，差异均有统计学意义（P均〈0.05），RT-PCR的检测结果表明wnt-2、β-catenin、TCF-4的翻译水平A、B、C、D组呈逐渐递减趋势，差异均有统计学意义（P均〈0.05）。结论吸烟对机体wnt/β-catenin细胞转导途径具有激活作用，在经过香烟激活wnt/β-catenin信号通路的机体中肺鳞状细胞癌的发病率将大幅度提升，对吸烟人群wnt/β-catenin信号转导情况进行检测可对肺鳞状细胞癌的发生有一定的指示作用。
ObjectiveTo investigate the correlation of smokers in wnt / β-catenin signaling pathway activation as well as their correlation with lung squamous cell carcinoma incidence.MethodsIn the Department of Respiratory Medicine of the People＇s Hospital of Wuhan University, 30 cases lung squamous cell carcinoma patients with a history of smoking, 30 cases lung squamous cell carcinoma patients with no history of smoking, 30 cases bronchiectasis patients with a history of smoking, 30 cases bronchiectasis patients with no history of smoking were chose respectively as A group, B group, C group and D group.After the patients were treated by surgery, the removal of diseased tissue used immunohistochemical staining for CD68 ＋ testing, TNF-α, wnt-2, β-catenin and the expression of TCF-4 were measured by using Western blot hybridization method, then wnt-2, β-catenin, TCF-4 transcription of mRNA concentrations were detected by using RT -PCR method.ResultsImmunohistochemical studies showed that cells in TNF-α, CD68 ＋ expression of A group, B group, C group and D group were respectively 0.895±0.036 and 0.931±0.237, 0.125±0.018 and 0.631±0.064, 0.083±0.042 and 0.311±0.062, 0.053±0.004 and 0.196±0.020, the differences were statistically significant（P〈0.05）. Westblot test Results showed that wnt-2, β-catenin, TCF-4 expression level showed a gradually decreasing trend, the difference was statistically significant（P〈0.05）, testing of RT-PCR showed that wnt-2, β-catenin, TCF -4 translation level showed a gradually decreasing trend, the difference was statistically significant（P〈0.05）.ConclusionSmoking for body wnt / β-catenin pathway has a cell activation, after cigarette activate wnt / β-catenin signaling pathway in the incidence of lung squamous cell carcinoma of the body will greatly enhance the smoking crowd wnt / β-catenin signaling transduction circumstances.The detection of wnt/ beta -catenin signal transduction in smokers can be used to indicate the occurrence of lung squamous cell carcinoma.
出处
《中国综合临床》
2017年第2期149-154,共6页
Clinical Medicine of China
