摘要
目的:探究单味及多味中药灌洗对鼻咽癌放疗后鼻腔、鼻咽组织的修复作用。方法:将80只豚鼠分为单纯照射组、对照组、鼻腔单味中药(铁皮石斛)灌洗组、多味中药中药灌洗组,每组20只。每组豚鼠用Co-60治疗机产生的射线作鼻部照射,照射开始当天即开始给药,对照组用生理盐水鼻腔;鼻腔单味中药灌洗组用新鲜铁皮石斛提取后的灌洗液灌洗;多味中药灌洗组用中药复方制剂吸入液对豚鼠鼻腔灌洗;对照组及单纯照射组不用药。采集总动脉处血液行肝肾功能检测,取鼻咽部黏膜HE染色后行光学显微镜观察细胞形态,原位免疫组织化学法(TUNEL法)检测药物对黏膜组织内细胞凋亡的影响,透射电镜及扫描电镜观察超微结构变化。结果:肝肾功能检测发现各组及多味中药灌洗组ALT、AST、AST/ALT、BUN、Cr水平有统计学差异(P<0.05);HE染色发现除对照组外,其余三组均呈不同程度的炎症状态;各TUNEL法发现各组均出现TUNEL阳性细胞,其中多味中药灌洗组TUNEL阳性细胞数最少,其次为单味中药灌洗组,对照组最多。电镜检查发现单味及多味中药灌洗组的纤毛倒伏、线粒体破坏等较对照组明显减少。结论:单味及多味中药灌洗法对于鼻咽癌放疗后损伤的鼻黏膜修复作用更加明显,能够促进炎症的消退,抑制细菌的生长。
Objective：To explore the refin-effect of single or complex Chinese medical herbs＇ lavage to nasal cavity and nasopharyngeal tissues after radio therapy to nasopharynx cancers.Methods：Eighty guinea pigs were divided into 4 groups including irradiated group, control group, treatment group with single Chinese medical herb and treatment group with complex Chinese medical herbs.The guinea pigs in each group irradiated with radiation therapy machine.Drugs were given the day when the irradiation was started.For the control group, irrigated the nasal cavity of guinea pigs with.For the treatment group with single Chinese medical herb, irrigate the nasal cavity of guinea pigs with extracting solution of dendrobium candidum.For the treatment group with complex Chinese medical herbs, irrigate the nasal cavity of guinea pigs with inhalation solution of complex Chinese patent medicine once a day for 4weeks.Collect the blood in common carotid artery irradiation, and tested the liver and kidney function.The nasopharyngeal mucosa was taken at the same time and was observed with HE staining and electron microscope, and detect the influence of drugs on cell apoptosis of mucosa tissue with TUNEL, and observed the change of ultrastructure by transmission electron microscopy or scanning electron microscope.Results：ALT、AST、AST/ALT、BUN and Cr were significantly different （P〈0.05） in this four groups through the test of liver.Through the test of TUNEL, we found that all the groups had TUNEL-positive cells, and the treatment group with complex Chinese medical herbs got the least number of it, then the treatment group with single Chinese medical herb, and the control group got the largest number of it.The phenomena of cilia lodging and mitochondrial damage were significantly reduce compared with the control group by electron microscopy.Conclusion：The lavage method of single or complex Chinese medical herbs to nasal cavity and nasopharyngeal tissues after radio therapy to nasopharynx cancers was significantly effective.And it can promote the resolution of inflammation, and inhibit the growth of bacteria.
出处
《陕西中医》
2017年第4期534-536,共3页
Shaanxi Journal of Traditional Chinese Medicine
基金
广西科学研究与技术开发计划项目(桂林攻13349008)
关键词
鼻咽肿瘤
动物实验
豚鼠
Nasopharyngeal neoplasms
Animal experimentation
Guinea pigs
