摘要
目的通过有效成分转移率及抗炎镇痛活性实验筛选复方柴胡葛根颗粒药材的最佳提取工艺。方法以HPLC法测定黄芩苷、葛根素、连翘苷复方柴胡葛根颗粒药材不同提取工艺的活性成分转移率;以小鼠耳肿胀实验以及醋酸致小鼠扭体实验考察复方柴胡葛根药材不同提取工艺的抗炎镇痛药效。结果工艺4为最优工艺,即将羌活、柴胡、连翘三味药材采用水蒸气蒸馏法提取挥发油;滤渣与板蓝根、桔梗一同煎煮3次,合并煎煮液,浓缩得流浸膏。另将葛根、黄芩采用60%乙醇提取,醇提取液浓缩得流浸膏。合并两部分流浸膏,加入挥发油。结论活性成分转移率实验和抗炎镇痛活性实验筛选得到的复方柴胡葛根颗粒提取工艺科学、合理、可行,适合现代化中药制剂的研究与开发。
Objective To screen the best extraction technology of fufangchaihugegen granules by evaluating the transfer rate of active constituent, and anti-inflammatory analgesic activity. Methods HPLC method was used to investigate the transfer rate of baicalin, puerarin, and forsythin under different extraction processes. The ear swelling rate of mice and acetic acid-induced writhing test in mice were used to investigate the anti-inflammatory analgesic activity of fufangchaihugegen granules under different extraction processes. Results The best extraction technology is NO.4. The volatile oil of notopterygium, bupleuri, and forsythia was extracted by steam distillation method. The residue was decocted three times, combined with isatis root and platycodon grandiflorum to obtain liquid extract. Then puerarin and baicalin were extracted by 60% alcohol, and concentrated it as liquid extract by alcohol. The two-part flow extract was combined and the volatile oil was added. Conclusion The extraction process of fufangchaihugegen granules screened by the active component transfer rate experiment and the anti-inflammatory analgesic activity is scientific, reasonable, and feasible. It is suitable for the research and development of the traditional Chinese medicine preparation.
出处
《中国药物警戒》
2017年第3期165-168,175,共5页
Chinese Journal of Pharmacovigilance
关键词
复方柴胡葛根颗粒
提取工艺
转移率
抗炎镇痛作用
fufangchaihugegen particles
extraction technology
transferring rate
anti-inflammatory and analgesic effects
