摘要
目的 探讨细胞核增殖抗原（Ki-67）、角蛋白7（CK7）和角蛋白20（CK20）在膀胱癌组织的表达及预后意义.方法 应用免疫组织化学链菌素抗生物素蛋白-过氧化物酶（SP）法检测138例膀胱癌组织石蜡切片中Ki-67、CK7和CK20的表达,并结合临床随访资料进行分析.结果 Ki-67、CK7和CK20在膀胱癌组织中均高表达,阳性率分别为82.6%、88.4%和78.3%.Ki-67在膀胱癌中的表达与分级（P=0.001）、分期（P=0.007）、肿瘤复发（P=0.044）和转移（P=0.037）显著相关;CK7在膀胱癌中的表达与分级（P=0.002）、分期（P=0.043）、转移（P=0.012）、组织学分型（P=0.020）显著相关;CK20在膀胱癌中的表达与患者性别（P=0.001）、分级（P=0.012）、分期（P=0.021）、肿瘤大小（P=0.034）及肿瘤复发（P=0.035）显著相关.Spearman等级分析表明Ki-67与CK20之间（P=0.026）及CK20与CK7之间（P=0.010）呈正相关,而Ki-67与CK7之间无明显相关（P=0.319）.Ki-67阳性及阴性患者的中位无进展生存期分别为10、21个月,Kaplan-Meier生存分析表明两者差异有统计学意义（P=0.000）;CK7阳性及阴性患者的中位无进展生存期分别为13、9个月,Kaplan-Meier生存分析表明两者差异无统计学意义（P=0.074）;CK20阳性及阴性患者的中位无进展生存期分别为9、18个月,Kaplan-Meier生存分析表明两者差异有统计学意义（P=0.000）.结论 Ki-67及CK20在膀胱癌组织中的表达随着肿瘤分级分期的增高而升高,CK7的表达随肿瘤分级分期的增高而降低,三者共同参与到膀胱癌的发生发展中.
Objective To investigate the expression of proliferation cell nuclear antigen （Ki-67）, cytokeratin-7 （CK7） and cytokeratin-20 （CK20） in bladder cancer and its prognostic significance.Methods The expression of Ki-67, CK20 and CK7 in 138 cases of bladder cancer tissue was detected by immunohistochemical SP method.The clinical follow-up data were analyze.Results Ki-67, CK7 and CK20 was highly expressed in bladder cancer tissues, with the positive rate of 82.6%, 88.4% and 78.3% respectively.The expression of Ki-67 in bladder cancer was significantly associated with grading （P=0.010）, staging （P=0.007）, recurrence （P=0.044） and metastasis （P=0.037）.The expression of CK7 in bladder cancer was significantly associated with grading （P=0.002）, staging （P=0.043）, metastasis （P=0.012） and histological type （P=0.020）.The expression of CK20 in bladder cancer was significantly associated with gender, grading, with gender （P=0.001）, grading （P=0.012）, staging （P=0.021）, tumor size （P=0.034） and tumor recurrence （P=0.035）.Spearman rank analysis showed that Ki-67 and CK20 had positive correlation （P=0.026）, so as CK20 and CK7 （P=0.010）.But Ki-67 and CK7 have no correlation （P=0.319）.The median progression-free survival of patients positive or negative for Ki-67 was 10 and 21 months, respectively.Kaplan-Meier survival analysis revealed that the difference was statistically significant （P=0.000）.The median progression-free survival of patients positive or negative for CK7 was 13 and 9 months, respectively.Kaplan-Meier survival analysis demonstrated that the difference was not statistically significant （P=0.074）.The median progression-free survival of patients positive or negative for CK20 was 9 and 18 months, respectively.Kaplan-Meier survival analysis illustrated that the difference was statistically significant （P=0.000）.Conclusion Along with the increase of tumor grade and stage, the expression of Ki-67 and CK20 in bladder cancer tissues was up-regulated, and CK7 down-regulated.All of them are involved in the advent and development of bladder cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2017年第3期497-499,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金（81570685）
关键词
膀胱癌
细胞核增殖抗原
角蛋白7
角蛋白20
预后
Bladder cancer
Proliferation cell nuclear antigen
Cytokeratin-7
Cytokeratin-20
Prognosis
