摘要
目的:观察穴位注射对变应性鼻炎(AR)大鼠鼻黏膜组织嗜酸性粒细胞(EOS)和嗜酸性粒细胞趋化因子(Eotaxin)表达的影响,探讨其治疗AR的作用机制。方法:SD大鼠随机分为正常组、模型组和穴注组,每组8只。采用卵清蛋白致敏诱发AR模型。穴注组于分组后第1、5、9、13天取双侧"迎香"穴及"印堂"穴进行穴位注射治疗,每穴注射转移因子、地塞米松、利多卡因混合液0.1mL,共4次。采用HE染色法高倍镜下计数鼻黏膜EOS,免疫组织化学法和荧光定量PCR法检测鼻黏膜组织中Eotaxin蛋白和mRNA的表达。结果:与正常组相比,模型组大鼠鼻黏膜EOS计数、鼻黏膜Eotaxin蛋白和mRNA表达明显升高(P<0.05);与模型组相比,穴注组鼻黏膜EOS计数、鼻黏膜Eotaxin蛋白和mRNA表达明显降低(P<0.05)。结论:穴位注射治疗可下调鼻黏膜Eotaxin蛋白和mRNA的表达,减少EOS的募集和局部浸润,从而减轻鼻黏膜组织的炎性损伤。
Objective To observe the effect of acupoint injection on eosinophils（EOS） counts and expression of eotaxin in nasal mucosa of allergic rhinitis （AR） rats, so as to reveal its mechanism underlying improving AR. Methods Twenty-four Sprague-Dawley （SD） rats were randomly divided into normal, model and acupoint injection groups （n = 8 in each group）. The AR model was established by intraperitoneat injection of ovalbumin sensitization. Bilateral ＂Yingxiang＂（LI 20） and ＂Yintang＂（GV 29） were selected for acupoint injection of the mixture solution of lidocaine, dexamethasone, and transfer factor （0.1 mL/acupoint） on the 1st , 5th , 9th, and 13th day after AR model established, a total of four times. EOS in the nasal mucosa was counted un- der light microscope after HE staining. Protein and mRNA expressions of eotaxin in the nasal mucosa were detected by immunohis- tochemical and RT-PCR methods, respectively. Results Compared with the normal group, EOS counts, protein and mRNA ex- pressions of eotaxin in the nasal mucosa were significantly higher in the model group （P〈0.05）. Compared with the model group, EOS counts, protein and mRNA expressions of eotaxin in the nasal mucosa were significantly lower in the acupoJnt injection group （P^0.05）. Conclusion Acupoint injection can reduce the nasal mucosa inflammation by suppressing the protein and mRNA expressions of eotaxin, decreasing the infiltration and gathering of EOS in the nasal mucosa.
作者
张元
侯珣瑞
李丽红
杨濛
梁飞红
ZHANG Yuan HOU Xun-rui LI Li-hong YANG Meng LIANG Fei-hong(Clinical Medical College of Guizhou Medical University, Guiyang 550004, China The A f fliated Hospital of Guizhou Medical University, Guiyang 550004)
出处
《针刺研究》
CAS
CSCD
北大核心
2017年第2期141-144,共4页
Acupuncture Research
基金
贵州省卫生厅中医药管理局计划(No.QZYY 2013-88)
贵州省科技厅科技合作计划[黔科合LH字(2015)7427号]
