摘要
目的:观察艾灸对胶原诱导性关节炎(CIA)大鼠血清白介素-17(IL-17)和肿瘤坏死因子-α(TNF-α)含量的影响,探讨艾灸对类风湿性关节炎的作用机制。方法:将Wistar大鼠随机抽取8只为空白组,剩余大鼠制备CIA模型。初次免疫乳剂采用鸡Ⅱ型胶原与弗氏完全佐剂制备,0.3mL乳剂(含0.3mg胶原)分别等量皮下注射于大鼠左足底部、尾根上和背部。初次免疫7d后,同法诱发继发免疫,继发免疫乳剂由鸡Ⅱ型胶原与弗氏不完全佐剂制备,总共造模21d。将造模成功的24只大鼠随机分为模型组、药物组和艾灸组,每组8只。艾灸组用小艾条距"足三里"和"昆仑"穴20mm处悬灸,每穴20min,共40min,1次/d,左右肢交替进行,连续干预10d。药物组用0.1mg/100g甲氨蝶呤氯化钠溶液灌胃,1次/5d,共2次。于造模前后及治疗后测量各组大鼠左踝关节直径、体质量;治疗结束后拍摄左足跗部X光片,ELISA法检测大鼠血清中IL-17和TNF-α的含量。结果:造模后,与空白组相比,模型组大鼠踝关节直径、血清IL-17和TNF-α含量升高(P<0.05),体质量下降(P<0.05),X线下可见足跗部关节软组织肿胀,关节间隙变窄,骨质破坏;干预后,与模型组相比,药物组和艾灸组大鼠踝关节直径、血清IL-17和TNF-α含量下降(P<0.05),体质量升高(P<0.05),足跗部软组织肿胀减轻,骨骼畸形好转;药物组和艾灸组相比,各指标差异均无统计学意义(P>0.05)。结论:艾灸对CIA大鼠具有与甲氨喋呤类似的作用,其机制之一可能是艾灸可降低CIA大鼠血清中IL-17和TNF-α的含量。
Objective To observe the influence of moxibustion on serum interleukin -17 （IL-17） and tumor necrosis fac- tor alpha （TNF-α） levels in collagen-induced arthritis （CIA） rats, so as to study its mechanism underlying improvement of rheuma- toid arthritis. Methods A total of 40 mate Wistar rats were used in the present study, and 8 rats were randomly selected as a normal control group. The other 32 rats were modeled. The primary immunity emulsion was made with mixed Type chicken col- lagen and complete Freund＇s adjuvant, and 0.3 mL emulsion （containing 0.3 mg collagen） was injected equally into left pelma, tail root and the back. Seven days after the primary immune, the same procedure was conducted to induce the secondary immuni- ty, and the emulsion was made with mixed Type Ⅱ chicken collagen and incomplete Freund＇s adjuvant. The whole course of mo- deling lasted 21 days. And then 24 CIA rats were randomly divided into model group, medication group and moxibustion group （n = 8 in each group）. For those of the moxibustion group, suspended moxibustion with 20 mm distance above ＂Zusanli＂（ST 36） and ＂Kunlun＂ （BL 60） was performed for 20 min/acupoint, once daily, alternately on left and right hind limbs for 10 consecutive days. For those of the medication group, gavage of methotrexate （0.1 mg/100 g） was administrated once every 5 days, and to- tally two times. Left ankle joint diameter and body weight were detected, and X-ray of left tarsus was observed in each group be- fore and after modeling or after treatment. Serum levels of IL-17 and TNF-α（ were determined by ELISA kits. Results After mod- eling, the left ankle diameter and serum concentrations of IL-17 and TNF-αincreased （P〈0. 05）, and the body weight decreased （P〈0. 05） in the model group compared to the control group, combined with the tarsus soft tissue swelling, joint space narro-wing, bone destruction seen from the tarsal X-ray. After intervention, the ankle diameter, the serum IL-17 and TNF-α levels de- creased （ P〈0.05）, and the body weight increased （P〈0.05） in both medication and moxibustion groups compared to the mod- el group; meanwhile the tarsus soft tissue swelling and the bone deformity turned to be improved. There were no significant differ- ences between the medication group and the moxibustion group in above mentioned indexes （P〉0.05）. Conclusion Moxi- bustion is effective in CIA rats, and the mechanism may be related to the reduction of serum IL-17 and TNF-α levels.
作者
殷媛
陈天帷
张瑞
马文珠
YIN Yuan CHEN Tian-wei ZHANG Rui MA Wen-zhu(School of Acu moxibustion and Tuina ,Beijing University of Chinese Medicine, Beijing 100029, China)
出处
《针刺研究》
CAS
CSCD
北大核心
2017年第2期149-152,177,共5页
Acupuncture Research
基金
北京中医药大学2015年度基本科研业务费项目(No.2015-JXS-350)
