摘要
目的:观察艾灸"肺俞""肾俞"对哮喘大鼠外周血T细胞及血清白介素的影响,探讨艾灸治疗哮喘的免疫学机制。方法:SD大鼠随机分为正常组、模型组、艾灸组,每组各10只。以卵白蛋白加氢氧化铝腹腔注射并给予雾化激发法制备哮喘模型。艾灸组大鼠间接灸双侧"肺俞""肾俞"穴,每次30min,每日1次,共治疗14d。用HE染色行肺组织病理学观察,用流式细胞仪检测全血CD 3^+、CD 4^+、CD 8^+T细胞水平,用酶联免疫吸附法检测血清免疫球蛋白E(IgE)、白细胞介素-1β(IL-1β)、IL-1受体拮抗剂(IL-1Ra)水平。结果:模型组大鼠支气管壁肌层增厚,黏膜水肿,大量炎性细胞浸润,部分肺泡结构破坏明显;艾灸组大鼠肺泡壁结构完整,细支气管形态规则,管腔内无黏液栓形成及上皮细胞脱落等现象。模型组全血CD 8^+、CD3^+T细胞水平及血清IgE、IL-1β含量较正常组显著升高(P<0.01)。艾灸组CD 8^+T细胞及血清IgE、IL-1β水平较模型组显著降低(P<0.05,P<0.01),CD 4^+T细胞水平、血清IL-1Ra水平较模型组显著升高(P<0.01,P<0.05)。结论:艾灸大鼠"肺俞""肾俞"穴能降低血清IgE、IL-1β水平,升高血清IL-1Ra水平,达到治疗哮喘的目的。
Objective To observe the effect of moxibustion at "Feishu" (BL 13) and "Shenshu'(BL 23) on peripheral blood T cells and serum interleukin in rats with asthma, so as to explore its immunological mechanisms in relieving asthma. Methods Thirty SD rats were randomly divided into normal, model and moxibustion groups(10 rats/group). Asthma model was established by intraperitoneal injection of ovalburnin and aluminum hydroxide, and ovalbumin inhalation. Moxibustion was applied to bilateral "Feishu" (BL 13) and "Shenshu"(BL 23) for 30 min, once daily for 14 d. The pathologic changes of the lung tissue were detected by H.E. staining. The levels of OD 3+ , CD 4+ , CD 8+ T cells in whole blood were detected by flow cytometry. Serum IgE, IL-1β, IL-1 Ra contents were assayed by ELISA (enzyme-linked immunosorbent assay). Results The thickened muscle of bronchial wall, mucosal edema, infiltration of inflamma- tory cells and partial destruction of the alveolar structure were found in the model group. While in the moxibustion group, the structure of the alveolar wall was complete, the morphology of the bronchioles was regular, and there were no mucus plug formation and epithelial cell shedding in the lumen. The contents of blood CD 8+ and CD 3+ T cells, and serum IgE and IL-1β in the model group were obviously higher than those in the normal group (P〈0.01). Compared with the model group, the levels of blood CD 8+ T cell, serum IgE and IL- 1β in the moxibustion group were all decreased (P〈0.05, P〈0. 01), meanwhile the levels of blood OD 4+ T cell and serum IL-1 Ra were increased ( P〈0.01, P〈0.05). Conclusion Moxibustion can reduce the levels of serum IgE and IL-1β, and increase the level of serum IL-1 Ra, which may play an important role in the treatment of asthma.
作者
陈向华
何海明
CHEN Xiang hua HE Hai-ming(Clinical College of Acupuncture-moxibustion and Orthopedic, Anhui University of Chi- nese Medicine, He f ei 230038, Chin)
出处
《针刺研究》
CAS
CSCD
北大核心
2017年第2期159-162,共4页
Acupuncture Research
基金
安徽中医学院科研项目(No.2012zr 010)