摘要
目的:探讨隔盐隔姜灸"神阙"穴对逼尿肌反射亢进的效应及其作用机制。方法:成年雌性SD大鼠30只,其中20只大鼠采用T9完全脊髓横断方法建立逼尿肌反射亢进模型后随机分为模型组(10只)和艾灸组(10只),余10只作为假手术组行假脊髓横断手术。艾灸组大鼠出现尿失禁(约造模2周)后,给以隔盐隔姜灸"神阙"穴治疗,每次灸3壮,每日1次,连续治疗7d。各组在干预结束后,进行尿流动力学检测,之后取膀胱逼尿肌,采用免疫蛋白印迹法检测M2、M3受体蛋白表达情况。结果:与假手术组大鼠相比,模型组大鼠排尿间期明显较短(P<0.01),最大膀胱内压明显较高(P<0.01),膀胱逼尿肌M2受体蛋白表达显著升高(P<0.05),M3受体蛋白表达无明显变化(P>0.05);与模型组相比,艾灸组大鼠排尿间期明显较长(P<0.01),最大膀胱内压明显降低(P<0.01),膀胱逼尿肌M2受体蛋白表达显著下降(P<0.05),而M3受体蛋白表达无明显变化(P>0.05);与假手术组相比,艾灸组以上指标差异均无统计学意义(均P>0.05)。结论:隔盐隔姜灸"神阙"穴能抑制脊髓横断大鼠的膀胱过度活动状态,其作用机制可能与减少膀胱逼尿肌M2受体蛋白的表达、抑制逼尿肌的过度收缩有关。
Objective To discuss the effects on detrusor hyperreflexia treated with ginger-salt-isolated moxibustion at＂Shenque＂（CV 8）and its mechanism.Methods Thirty female adult SD rats were selected.The model of detrusor hyperreflexia was prepared with complete spinal transection at T9,of which,20 rats were randomized into a model group（10rats）and a moxibustion group（10rats）.A sham-operation group（10rats）was set up for sham-spinal transection.In the moxibustion group,when urine incontinence occurred（about in 2weeks of modeling）,the ginger-salt-isolated moxibustion at＂Shenque＂（CV 8）was given,3moxa cones each time,once a day,continuously for 7days.After treatment,in each group,the urodynamic parameters were determined,after which,the bladder detrusor was collected.Western blot was used to determine the protein expressions of M2 and M3receptors.Results Compared with the sham-operation group,the micturition interval was shortened apparently（P〈0.01）;the maximal bladder pressure was increased apparently（P〈0.01）;the protein expression of M2 receptor in the detrusor was increased significantly（P〈0.05）and that of M3 receptor had no apparent change（P〈0.05）in the rats of the model group.Compared with the model group,the micturition interval was longer apparently（P〈0.01）,the maximal bladder pressure was reduced apparently（P〈0.01）,the protein expression of M2 receptor in the detrusor was reduced significantly（P〈0.05）and that of M3 receptor had no apparent change（P〉0.05）in the rats of the moxibustion group.Compared with the sham-operation group,the results of the above indicators were not different significantly in the moxibustion group（all P〉0.05）.Conclusion The ginger-salt-isolated moxibustion at＂Shenque＂（CV 8）suppresses the overactive bladder in the rat with spinal transection and its effect mechanism is possibly relevant with reducing the protein expression of detrusor M2 and inhibiting the excessive contraction of the detrusor.
出处
《中国针灸》
CAS
CSCD
北大核心
2017年第4期401-405,共5页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金资助项目:8140150462
北京市自然科学基金资助项目:7154206
北京市医院管理局青年人才培养"青苗"计划项目:QML20150904
关键词
逼尿肌反射亢进
隔盐隔姜灸
穴
神阙
尿动力参数
M2受体
M3受体
detrusor hyperreflexia
ginger-salt-isolated moxibustion
Point CV 8（Shenque）
uryodynamic parameters
M2 receptor
M3 receptor
